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Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen [1]. There is evidence showing that fibroblasts in different organs are intrinsically different [2]. Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. In addition, they secrete large quantities of hyaluronan in response to inflammatory stimuli [3].
RDF from ScienCell Research Laboratories are isolated from neonatal CD® IGS rat skin. RDF are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RDF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. RDF are negative for mycoplasma, bacteria, yeast, and fungi. RDF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing RDF in vitro.
Catalog No. | R2300 |
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Country of Manufacture | United States |
Product Code | RDF |
Size/Quantity | 5 x 10^5 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137. [2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981. [3] Stair S, Carlson KW, Shuster S, Wei ET, Stern R (2002) Mystixin peptides reduce hyaluronan deposition and edema formation. Eur J Pharmacol 30;450(3):291-6. |
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CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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2300 | HDF-f | Human Dermal Fibroblasts-fetal |
$492.00
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2320 | HDF-a | Human Dermal Fibroblasts-adult |
$258.00
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R2320 | RDF-a | Rat Dermal Fibroblasts-adult |
$394.00
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B2300 | BDF | Bovine Dermal Fibroblasts |
$427.00
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M2300-57 | MDF | Mouse Dermal Fibroblasts from C57BL/6 |
$433.00
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MEDIA | |||||
2301 | FM | Fibroblast Medium |
$115.00
As low as:
$70.00
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REAGENTS | |||||
0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
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0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
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0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
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0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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