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- Rat Embryonic Fibroblasts
Rat Embryonic Fibroblasts (REF) are used to support the growth of rat and human pluripotent stem cells [1]. REF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. REF are isolated from rat embryos and used at early passages [2]. To serve as feeder cells, REF must be treated with mitomycin C or by irradiation to prevent cell proliferation. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells.
REF from ScienCell Research Laboratories are isolated from embryonic day 14 CD® IGS rat embryos. These cells are cryopreserved at P0 and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume. REF are characterized by immunofluorescence with antibody specific to fibronectin. REF are negative for mycoplasma, bacteria, yeast, and fungi. REF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use DMEM (Cat. #09221) supplemented with 10% fetal bovine serum (FBS, Cat. #0010, 0025, 0500) for culturing REF in vitro.
| Catalog No. | R7540 |
|---|---|
| Country of Manufacture | United States |
| Product Code | REF |
| Size/Quantity | 1 x 10^6 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keepthe cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Bradley, A. (1987). Production and analysis of chimaeras. In Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, (ed. E. J. Robertson), pp. 113-151. Oxford: IRL Press. |
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R7540| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 09221 | DMEM | DMEM with High-Glucose, L-Glutamine and Sodium Pyruvate |
$18.00
|
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| REAGENTS | |||||
| 0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
|
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
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| 0133 | CFM | Cell Freezing Medium, 50 ml |
$48.00
|
||
| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
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| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
|
