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How can we help you today?
Need help with product selection and ordering?
Email us at sales@sciencellonline.com
Need help with scientific inquiries, technical assistance, product applications, and use?
Email us at techsupport@sciencellonline.com
Need help with issues concerning this website?
Email us at webmaster@sciencellonline.com
Have any marketing related inquiries?
Email us at marketing@sciencellonline.com
Need help ordering from abroad?
As the largest cell provider, we are available worldwide.
Please visit our Distributors page to select the company nearest to your location.
We are located at 1610 Faraday Ave, Carlsbad, CA 92008
Call us at (877) 602-8549 or (760) 602-8549
Fax us at (760) 602-8575
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Rat Embryonic Fibroblasts (REF) are used to support the growth of rat and human pluripotent stem cells. REF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. REF are isolated from rat embryos and used at early passages. To serve as feeder cells, REF must be treated with mitomycin C or by irradiation to prevent cell proliferation. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells.
REF from ScienCell Research Laboratories are isolated from embryonic day 14 CD® IGS rat embryos. These cells are cryopreserved at P0 and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume. REF are characterized by immunofluorescence with antibody specific to fibronectin. REF are negative for mycoplasma, bacteria, yeast, and fungi. REF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use DMEM (Cat. #09221) supplemented with 10% fetal bovine serum (FBS, Cat. #0500) for culturing REF in vitro.
Catalog No. | R7540 |
---|---|
Country of Manufacture | United States |
Product Code | REF |
Size/Quantity | 1 x 10^6 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keepthe cells in liquid nitrogen until cell culture is needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Bradley, A. (1987). Production and analysis of chimaeras. In Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, (ed. E. J. Robertson), pp. 113-151. Oxford: IRL Press. |
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We are an expanding biotechnology company whose mission is the research and development of cell and cell-related products for experimental use. About Sciencell