Rat IGF-2 ELISA Kit

Insulin-like growth factor-2 (IGF-2), also known as somatomedin A, is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-2 is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-2 gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting. IGF-2 appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland. ScienCell's rat IGF-2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat IGF-2-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The rat specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added, and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat IGF-2 captured in strips .

Catalog No.	EK0380
Country of Manufacture	China
Product Code	rIGF-2-ELISA
Size/Quantity	96 Tests in 8 x 12 divisible strips
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.
Shipping Info	Gel ice.
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