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- Rat Perineurial Fibroblasts
Perineurial fibroblasts are of mesenchymal origin and form the perineurium. The perineurium plays an important role in maintaining the integrity of the internal peripheral nerve environment by creating a physical barrier that, under physiologic condition, limits the entry of biologically active proteins, infectious agents, and blood-borne cells into the nerve bundles [1]. The perineurial fibroblasts are characterized by distinct ultrastructural features, including non-branching thin cytoplasmic processes coated by an external lamina and joined at their ends by a tight junction, few organelles, actin and vimentin filaments, and numerous pinocytotic vesicles [2]. Perineurial fibroblasts are initially recruited from the surrounding mesenchyme to form a loose, permeable sheath around axons and Schwann cells, where they are separated by the extracellular matrix. These cells later undergo a mesenchymal-to-epithelial transition to form tight junctions and organize into the perineurium. Perineurial fibroblasts are immunoreactive for vimentin and epithelial membrane antigen but not for the Schwann cell marker S-100 [3].
RPNF from ScienCell Research Laboratories are isolated from postnatal day 8 CD® IGS rat sciatic nerve. RPNF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RPNF are characterized by immunofluorescence with antibodies specific to vimentin and fibronectin. RPNF are negative for mycoplasma, bacteria, yeast, and fungi. RPNF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing RPNF in vitro.
Catalog No. | R1710 |
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Country of Manufacture | United States |
Product Code | RPNF |
Size/Quantity | 5 x 10^5 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Salzer, J. L. (1999) Creating barriers: a new role for Schwann cells and desert hedgehog. Neuron 22:627629. [2] Erlandson, R. A. (1991) The enigmatic perineurial cell and its participation in tumors and in tumor like entities. Ultrastruct Pathol. 15:335-351. [3] Ariza, A., Bilbao, J. M. and Rosai, J. (1988) Immunohistochemical detection of epithelial membrane antigen in normal and perineurial cells and perineurioma. Am J Surg Pathol. 12:678-683. |
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CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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1710 | HPNC | Human Perineurial Cells |
$754.00
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M1710-57 | MPNF | Mouse Perineurial Fibroblasts from C57BL/6 |
$444.00
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M1710 | MPNF | Mouse Perineurial Fibroblasts from CD1 |
$405.00
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REAGENTS | |||||
0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
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0103 | T/E | Trypsin/EDTA Solution (0.25%; 100ml) |
$21.00
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0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
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0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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