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How can we help you today?
Need help with product selection and ordering?
Email us at sales@sciencellonline.com
Need help with scientific inquiries, technical assistance, product applications, and use?
Email us at techsupport@sciencellonline.com
Need help with issues concerning this website?
Email us at webmaster@sciencellonline.com
Have any marketing related inquiries?
Email us at marketing@sciencellonline.com
Need help ordering from abroad?
As the largest cell provider, we are available worldwide.
Please visit our Distributors page to select the company nearest to your location.
We are located at 1610 Faraday Ave, Carlsbad, CA 92008
Call us at (877) 602-8549 or (760) 602-8549
Fax us at (760) 602-8575
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Pulmonary alveolar epithelial cells, comprised of alveolar type I and type II epithelial cells, line more than 99% of the internal surface area of the lung. Type I cells are large squamous cells whose thin cytoplasmic extensions cover >95% of the internal lung surface. They contain aquaporins and exhibit the highest osmotic water permeability of any mammalian cell type. Type II cells, which cover 2-5% of the internal lung surface, produce, secrete, and recycle pulmonary surfactant. The currently accepted hypothesis is that Type II cells maintain pulmonary fluid homeostasis by regulating active Na+ transport in the lungs, whereas Type I cells are "inert" cells that provide only a barrier function. A recent study, however, suggests that Type I cells may also regulate ion and fluid transport.
RPAEpiC from ScienCell Research Laboratories are isolated from rat lung. RPAEpiC are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. RPAEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18. RPAEpiC are negative for, mycoplasma, bacteria, yeast, and fungi. RPAEpiC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories. This cell type, however, is not recommended for expanding or long term cultures since the cells would differentiate to type I alveolar epithelial cells immediately after plating and type I alveolar epithelial cells do not proliferate in culture.
Recommended Medium
It is recommended to use Alveolar Epithelial Cell Medium (AEpiCM, Cat. #3201) for culturing RPAEpiC in vitro.
Catalog No. | R3200 |
---|---|
Country of Manufacture | United States |
Product Code | RPAEpiC |
Size/Quantity | 1 x 10^6 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Crapo, J. D., Young, S. L., Fram, E. K., Pinkerton, K. E., Barry, B. E., & Crapo, R. O. (1983) Morphometric
characteristics of cells in the alveolar region of mammalian lungs. Am. Rev. Respir. Dis. 128:S42-S46. [2] Wright, J. R. & Dobbs, L. G. (1991) Regulation of pulmonary surfactant secretion and clearance. Annu. Rev. Physiol. 53:395-414. [3] Johnson, M. D., Widdicombe, J. H., Allen, L., Barbry, P. and Dobbs, L. G. (2002) Alveolar epithelial type I cells contain transport proteins and transport sodium, supporting an active role for type I cells in regulation of lung liquid homeostasis Proc. Natl. Acad. Sci. USA. 99(4):1966-1971.. |
No Publication available at this time
CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
---|---|---|---|---|---|
MEDIA | |||||
3201 | AEpiCM | Alveolar Epithelial Cell Medium |
$135.00
As low as:
$90.00
|
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