Ready-To-Use 3D Human Mesenchymal Stem Cell Spheroids

Mesenchymal stem cells (MSCs) have emerged as a leading candidate for use in cell therapy due to its multifunctional properties. Their ability to differentiate into specific cell types, their immune-suppressive properties, as well as their potent tropic effects during tissue repair make them invaluable in cell therapy [1]. Several lines of evidence has shown that culturing MSC as three-dimensional (3D) spheroids enhances a wide range of biological properties [1-3]. Traditionally, the generation of 3D MSC spheroids can be tedious and rate limiting. In order to facilitate high-throughput MSC studies, ScienCell offers ready-to-use 3D mesenchymal stem cell spheroids (SP3D-HMSCS), which can be thawed and plated directly in multiwell plates. By RT-qPCR analysis, we demonstrate that SP3D-HMSCS have elevated mRNA expression of pluripotency markers such as Sox2, Nanog, and CD90, indicating the enhanced stemness. Furthermore, chemotaxic factor (CXCR4), anti-inflammatory molecule (PEG2,), as well as other endogenous growth factors (VEGF and FGF2) are highly expressed in 3D MSC spheroids, thereby promoting the migration, colonization, and viability of MSC. SP3D-HMSCS are, therefore, a great model for examining the functions of mesenchymal stem cells in cell therapy and tissue regeneration.