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How can we help you today?
Need help with product selection and ordering?
Email us at sales@sciencellonline.com
Need help with scientific inquiries, technical assistance, product applications, and use?
Email us at techsupport@sciencellonline.com
Need help with issues concerning this website?
Email us at webmaster@sciencellonline.com
Have any marketing related inquiries?
Email us at marketing@sciencellonline.com
Need help ordering from abroad?
As the largest cell provider, we are available worldwide.
Please visit our Distributors page to select the company nearest to your location.
We are located at 1610 Faraday Ave, Carlsbad, CA 92008
Call us at (877) 602-8549 or (760) 602-8549
Fax us at (760) 602-8575
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Coronaviruses are a family of large RNA viruses with size ranging from 26 to 32 kb. These viruses are zoonotic and in humans can cause respiratory infections. As the coronavirus is an RNA virus, it has a relatively high mutation rate resulting in rapid evolution. In December 2019, a new deadly coronavirus known as SARS-CoV-2, which has a high sequence similarity to SARS-CoV, was identified as the cause of the Covid-19 outbreak. Since then, numerous variants of SARS-CoV-2 were reported around the world. Evidence has shown that some S gene mutations have the potential to increase transmissibility by altering the interactions with human angiotensin-converting enzyme 2 (ACE2) gene, which serves as a cellular receptor for SARS-CoV-2. It is critical, therefore, to monitor the mutations in the S gene of SARS-CoV-2 viruses.
ScienCell's SARS-CoV-2 S Gene PCR+Sanger Sequencing Kit (SSGPS) provides a convenient and fast way to sequence the entire S gene of SARS-CoV-2 coronavirus in samples. Included in the kit are four amplification primer sets (SSG-1F/1R, 2F/2R, 3F/3R, and 4F/4R, see Figure 1), which can amplify the four regions that together cover the entire SARS-CoV-2 S gene. Linked to each of the amplification primers is a sequencing primer (forward or reverse, see Figure 1), therefore, all four PCR products can be conveniently sequenced by the forward and reverse sequencing primers that are included in the kit.
Figure 1. Primer binding locations on the SARS-CoV-2 S gene.
Catalog No. | RU7158 |
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Country of Manufacture | United States |
Product Code | SSGPS |
Size/Quantity | 40 samples |
Product Use | For Research Use Only. Not for use in diagnostic procedures. |
Storage | Upon receipt, store the primers, RubyNStart One-step RT-PCR master mix, and RubyNStart One-step RT-PCR enhancer at -20°C in a manual defrost freezer. |
Shipping Info | SSGPS is shipped on dry ice. |
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