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- Telomerase Activity Quantification qPCR Assay Kit
Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes from degradation and genetic information loss. Telomerase is an RNA-containing enzyme that synthesizes the telomere repeat sequence at the 3' end of telomeres. Normal diploid cells lack telomerase activity, therefore, telomeres are lost with each cell cycle. In certain cells such as embryonic stem cells and most cancer cells, however, telomerase is activated to maintain telomere length and cellular immortality. The quantitative detection of telomerase activity is important in many aspects of cell biology, such as stemness and oncogenesis.
ScienCell's Telomerase Activity Quantification qPCR Assay Kit (TAQ) is designed to quantitatively compare telomerase activity among cell populations. The cell lysis buffer is a mild lysis buffer that enables the release of telomerase in the native state. The telomere primer set (TPS) recognizes and amplifies newly synthesized telomere sequences in the assay. The carefully designed primers ensure at suggested assay conditions: (i) high efficiency for trustworthy quantification; and (ii) no non-specific amplification. The primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.
| Catalog No. | 8928 |
|---|---|
| Country of Manufacture | United States |
| Product Code | TAQ |
| Size/Quantity | 100 reactions |
| Product Use | This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Please refer to the product sheet |
| Shipping Info | Dry ice |
8928-
It worked! Review by Krys
Ease-of-use Overall quality of the product Technical resources We used this product to determine whether our compound of interest had a stimulatory effect on telomerase activity in Chinese Hamster Ovary (CHO) telomerase-positive cells. And it worked. We saw a clear difference between the telomerase-positive and telomerase-negative controls when run on our BioRad IQ qPCR instrument.
(Posted on 9/22/2022)
1.) Yuan M, Eberhart C, Pratilas C, Blakeley J, Davis C, Stojanova M, Reilly K, Meeker A, Heaphy C, Rodriguez F. (2022) Therapeutic vulnerability to ATR inhibition in concurrent NF1 and ATRX-deficient high-grade solid tumors. bioRxiv preprint.
2.) Li Z, Li W, Zhou D, Zhao J, Ma Y, Huang L, Dong C, Wilson J, Huang G. (2021) Alleviating oxidative damage-induced telomere attrition: a potential mechanism for inhibition by folic acid of apoptosis in neural stem cells. Research Square preprint.
3.) Lu H, Lyu Y, Tran L, Lan J, Xie Y, Yang Y, Murugan NL, Wang YJ, Semenza GL. (2021) HIF-1 recruits NANOG as a coactivator for TERT gene transcription in hypoxic breast cancer stem cells. Cell Reports. 36(13)
4.) Dratwa M, Wysoczanska B, Turlej E, Anisiewicz A, Maciejewska M, Wietrzyk J, Bogunia-Kubik K. (2020) Heterogeneity of telomerase reverse transcriptase mutation and expression, telomerase activity and telomere length across human cancer cell lines cultured in vitro. Experimental Cell Research. 396(1)
5.) Nevi L, Costantini D, Safarikia S, Di Matteo S, Melandro F, Berloco PB, Cardinale V. (2019) Cholest-4,6-Dien-3-One Promote Epithelial-To-Mesenchymal Transition (EMT) in Biliary Tree Stem/Progenitor Cell Cultures In Vitro. Cells. 8(11)
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
