Total Glutathione Assay

Reduced glutathione (GSH) is a tripeptide (g-glutamylcysteinylglycine) and plays a key role in preventing oxidative damage in living organisms. GSH is commonly the most abundant low molecular mass thiol in living cells. Intracellular GSH status appears to be a sensitive indicator of the redox state of a cell. High levels of GSH has been proven to correlate with good health and longevity, whereas low levels of GSH are believed to contribute to many medical conditions, such as heart attack, neurodegenerative diseases. This colorimetric assay is based on the reaction between 5’, 5’-Dithiobis 2-nitrobenzoic acid (DTNB) and GSH to form TNB, which exhibits maximum absorbance at 412 nm. The intensity of the absorbance is proportional to the GSH level in the sample. Since glutathione reductas