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All-Inclusive 3D Human Hepatic Stellate-Endothelial Cell Spheroid Formation Kit

Immunostaining of the co-culture spheroids with the stellate cell markers (Vimentin and Smooth Muscle Actin) and the endothelial cell marker Von Willebrand Factor.

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All-Inclusive 3D Human Hepatic Stellate-Endothelial Cell Spheroid Formation Kit

Catalog #3D-5000

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ScienCell has developed the all-inclusive 3D human hepatic stellate-endothelial cell spheroid formation kit (3D-HHSteECSF) comprised of human hepatic stellate cells and sinusoidal endothelial cells. This kit is designed for studying the cellular crosstalk between hepatic stellate cells and sinusoidal endothelial cells during liver diseases such as liver fibrosis and caner metastasis.

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$1,808.00

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Product Description
Specifications
Technical Resources
Product Reviews
Publications

Product Description

Liver fibrogenesis and angiogenesis are closely linked processes. For example, in physiological conditions, hepatic sinusoidal endothelial cells (HSEC) maintain hepatic stellate cell (HSteC) quiescence, thereby inhibiting intrahepatic vasoconstriction and fibrosis development. When capillarized, however, hepatic sinusoidal endothelial cells lose their capacity to inactivate hepatic stellate cells, thus promoting fibrogenesis and intrahepatic vasoconstriction. To investigate the signaling crosstalk between these cellular events, ScienCell has developed the all-inclusive 3D human hepatic stellate-endothelial cell spheroid formation kit (3D-HHSteECSF) comprised of human hepatic stellate cells and sinusoidal endothelial cells. Furthermore, immunostaining of the co-culture spheroids reveals the presence of vimentin-positive stellate cells and von willebrand factor (VWF)-positive sinusoidal endothelial cells. The spheroids also contain the activated stellate cell population marked by the smooth muscle actin (SMA) staining.

Kit Components:

3D Cell Culture Components
Cat #	# of vials	Product Name	Quantity	Storage
5000	1	Human Hepatic Sinusoidal Endotdelial Cells (HHSEC)	5 × 10^5 cells	Liquid nitrogen
5300	1	Human Hepatic Stellate Cells (HHSteC)	5 × 10^5 cells	Liquid nitrogen
3D-5201	1	3D-Hepatic Spheroid Medium (3D-HSpM)	200 mL	2-8 °C
3D-5452	1	3D-Hepatic Stellate-Endothelial Spheroid Supplement (3D-HSteESpS)	4 mL	-20 °C
0583	1	Penicillin/Streptomycin Solution (P/S)	2 mL	-20 °C
0343 (or) 0353 (or) 0383	2	Ultra-Low Binding Culture Plates (24-, 48-, or 96- well plate)	2 plates	RT
2D Cell Culture Components
Cat #	# of vials	Product Name	Quantity	Storage
8711	1	2D-Hepatic Stellate-Endothelial Cell Co-culture Medium (2D-HSteECM)	500 mL	2-8 °C
8762	1	2D-Hepatic Stellate-Endothelial Cell Co-culture Growth Supplement (2D-HSteECGS)	5 mL	-20 °C
0025	1	Fetal Bovine Serum (FBS)	25 mL	-20 °C
0503	1	Penicillin/streptomycin Solution (P/S)	5 mL	-20 °C

Catalog No.	3D-5000
Country of Manufacture	United States
Product Code	3D-HHSteECSF
Size/Quantity
Product Use	3D-HHSteECSF are for research use only. It is not approved for human or animal use, or application in clinical or in vitro diagnostic procedures.
Storage
Shipping Info	5000, 5300, 3D-5452, 0583, 8762, 0025, and 0503 are shipped on dry ice. 3D-5201, 8711, and (0343 or 0353 or 0383) are shipped at room temperature.

Specifications Technical Resources Product Reviews Publications

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SP3D-5300	SP3D-HSteCS	Ready-to-use 3D Hepatocyte-Stellate Cell Spheroids	$1,808.00