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GeneQuery™ HLA-A PCR+Sanger SBT Typing Kit

Catalog #GK891

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GeneQuery™ HLA-A PCR+Sanger SBT Typing Kit (SBT-A) utilizes the Sequence-Based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.

Kit Components:

Cat #   Component Quantity Storage
GK891a HLA-A amplification primer set (A_ampF and A_ampR) 400 µL -20°C
GK891b A_2F sequencing primer mix, 10 µM 200 μL -20°C
GK891c A_2R sequencing primer mix, 10 µM 200 μL -20°C
GK891d A_3F sequencing primer mix, 10 µM 200 μL -20°C
GK891e A_3R sequencing primer mix, 10 µM 200 μL -20°C
GK891f A_4F sequencing primer mix, 10 µM 200 µL -20°C
GK891g A_4R sequencing primer mix, 10 µM 200 μL -20°C
GK891h Nuclease-free H2O 4 mL 4°C
GK891r Human genomic DNA reference (GDR), ~50 ng/µL
(HLA-A*02:01 and HLA-A*25:01)
10 μL -20°C
MB6038-1 2X EmeraldNStart HiFi Marathon PCR Master Mix, 1 mL 2 vials -20°C

Availability: In stock

$595.00
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