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GeneQuery™ HLA-DRB1 PCR+Sanger SBT Typing Kit

Catalog #GK896

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GeneQuery™ HLA-DRB1 PCR+Sanger SBT Typing Kit (SBT-DRB1) utilizes the Sequence-based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.

Kit Components:

Cat #   Component Quantity Storage
GK896a HLA-DRB1 amplification primer set (R_ampF and R_ampR) 400 µL -20°C
GK896b R_2F sequencing primer mix, 10 µM 200 μL -20°C
GK896c R_2R sequencing primer mix, 10 µM 200 μL -20°C
GK896d R_3F sequencing primer mix, 10 µM 200 μL -20°C
GK896e R_3R sequencing primer mix, 10 µM 200 μL -20°C
GK896f R_4F sequencing primer mix, 10 µM 200 µL -20°C
GK896g R_4R sequencing primer mix, 10 µM 200 μL -20°C
GK896h Nuclease-free H2O 4 mL 4°C
GK896r Human genomic DNA reference (GDR), ~50 ng/µL
(HLA-DRB1*03:01 and HLA-DRB1*15:01)
10 μL -20°C
MB6038-1 2X EmeraldNStart HiFi Marathon PCR Master Mix, 1 mL 2 vials -20°C

Availability: In stock

$595.00
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