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- GeneQuery™ Human B and T Cells Development Markers qPCR Array Kit
ScienCell's GeneQuery™ Human B and T Cells Development Markers qPCR Array Kit (GQH-BTD) is designed to facilitate gene expression profiling of 40 marker genes involved in the differentiation of common lymphoid progenitors (CLPs) into B and T cells. Brief examples of how included genes may be grouped according to their functions are shown below:
- CLP markers: MME, CD34, IL7R, FLT3
- ProB-PreB-immature B cell markers: MME, MS4A1, SPN, PTPRC, IL7R, CD79A/B, CD40, CD19
- B1 cell markers: CD19, MS4A1, CD27, SPN, CD40LG
- Regulatory B cell markers: CD1D, CD5, CD19, CR2, CD24
- Marginal Zone B cell markers: CD1C, CD19, MS4A1, CR2, CD24
- Follicular B cell markers: CD19, MS4A1, CR2, CD22, FCER2
- Memory B cell markers: CD27, CD80, CD84, CD86
- Plasmablast-plasma cell markers: CD19, CD27, CD38, IL6R, SDC1, SLAMF7
- Double-negative and double-positive stage T cell markers: CD1A, CD3D/E/G, CD4, CD5, CD7, CD8A/B, CD38, SELE/L/P, CD160, CD200
Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
Catalog No. | GK116 |
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Country of Manufacture | United States |
Product Code | GQH-BTD |
Size/Quantity | One 96-well plate, one 20ul reaction per well |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
Shipping Info | Ambient temperature. |
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