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- GeneQuery™ Human HIF1 Signaling Response qPCR Array Kit
ScienCell's GeneQuery™ Human HIF1 Signaling Response qPCR Array Kit (GQH-HFT) is designed to facilitate gene expression profiling of 88 key genes involved in HIF1 downstream signaling upon hypoxia-induced activation. HIF1 activity is a major component of the human response to hypoxia. Brief examples of how included HIF1 downstream genes may be grouped according to regulatory function are shown below:
- Cell cycling: DHFR, MAP3K5, POLE2, PRC1, SKP2
- Extracellular matrix remodeling: COL1A1, COL5A1, LOX, P4HA1, PLOD1
- Angiogenesis: HMOX1, MMP2, VEGFA, ANGPTL4, PGF
- Metabolism: PKM, STC2, DHX35, DDX11, ERO1A
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
| Catalog No. | GK036 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-HFT |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
GK036No Publication available at this time
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 3110 | HPASMC | Human Pulmonary Artery Smooth Muscle Cells |
$748.00
|
||
| 3300 | HPF | Human Pulmonary Fibroblasts |
$645.00
|
||
| 3114 | HPASMC cDNA | Human Pulmonary Artery Smooth Muscle Cell cDNA |
$453.00
|
||
| 3304 | HPF cDNA | Human Pulmonary Fibroblast cDNA |
$453.00
|
||
| 8014 | HUAEC cDNA | Human Umbilical Artery Endothelial Cell cDNA |
$453.00
|
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| MEDIA | |||||
| 1001 | ECM | Endothelial Cell Medium |
$139.00
As low as:
$92.00
|
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| 1101 | SMCM | Smooth Muscle Cell Medium |
$126.00
As low as:
$86.00
|
||
| 8010 | HUAEC | Human Umbilical Artery Endothelial Cells |
$477.00
|
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| GK017 | GQH-ANG | GeneQuery™ Human Angiogenesis qPCR Array Kit |
$286.00
|
