GeneQuery™ Human Inflammatory Cytokines and Receptors qPCR Array Kit

ScienCell's GeneQuery™ Human Inflammatory Chemokines, Interleukins, and Receptors qPCR Array Kit (GQH-CHE) surveys 88 key genes involved in the first steps of inflammation signaling.  Inflammation occurs as a protective response against harmful stimuli, as a signal to initiate tissue repair, and involves the local vascular system.  Cytokines are an important facet of the initial inflammatory response and include chemokines, interleukins, lymphokines, and tumor necrosis factors.  This kit focuses on tumor necrosis factors (TNFs), interferons, and the initiation of a local vascular response.  Note: For a survey of 88 additional cytokines involved in the initial inflammatory response, a separate GeneQuery array is available (GK110; Human Inflammatory Chemokines, Interleukins, and Receptors).  Brief examples of how included genes may be categorized are shown below:

• Interferon signaling: IFNA1, IFNA21, IFNAR1, IFNB1, IFNG
• TNF signaling: TNF, TNFRSF10A, TNFRSF14, TNFSF10, TNFSF8
• Vascular response signaling: GDF2, GPI, MDK, TYMP, VEGFA
• Growth factors: FGF1, FGF2, GDF3, PDGFB, VEGFD
• Other cytokines: CSF1, EPO, LTA, NAMPT, SPP1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.