GeneQuery™ Human Oncogenes qPCR Array Kit

ScienCell's GeneQuery™ Human Oncogenes qPCR Array Kit (GQH-ONC) surveys a panel of 88 known oncogenes to assist in cancer research.  Oncogenes are genes with the potential to transform cells after a gain-of-function mutation. Brief examples of how genes may be grouped according to their functions are shown below:     

• Transcription factors: ATF1, CDX2, ELK4, ETS1, JUN, JUNB, MYB, MYC, NCOA4, NFKB2, PPARG, REL, SOX10, STAT3
• Growth factor signaling: EGFR, ERBB2, FGFR1, FGFR2, PDGFB
• Protein kinases/Signal transduction: AKT1, BRAF, HRAS, KRAS, LMO2, SMO, PIK3CA, RAF1, RET, ROS1, WNT1
• Cell cycling/DNA repair/Growth regulation: ABL1, BAX, BCL2, CARD11, CCND1,  CCNE1, CDK4, HMGA2, MDM2, PLAG1, PTPN11
• Other: Protein turnover/mRNA regulation/Scaffolding: CBLB, DDX6, FUS, GOPC, KIT, KMT2A, MECOM, SS18, TET2, TFG

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.