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- GeneQuery™ Human Shear Stress and Mechanotransduction qPCR Array Kit
ScienCell's GeneQuery™ Human Shear Stress and Mechanotransduction qPCR array kit (GQH-SSM) is designed to facilitate gene expression profiling of 88 key endothelial cell genes responsive to shear stress and mechanotransduction. Brief examples of how included genes may be grouped according to function are shown below:
- Signal transduction: CAV1, IP3R, MAPK1, PIAS1, SENP2
- ECM/cytoskeletal remodeling: COL4A2, DNM1, LAMA4, PMP22, VCAM1
- Immune response: IL13RA1, NFKB1, SELE, PTGS2, TLR2
- Transcription factors: GATA4, NCOR2, SP1, TRA2B, PPARG
- Ion transport: CCL14, NUP62, PIEZO1
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
| Catalog No. | GK024 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-SSM |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
GK024No Publication available at this time
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 1000 | HBMEC | Human Brain Microvascular Endothelial Cells |
$905.00
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| 8004 | HUVEC cDNA | Human Umbilical Vein Endothelial Cell cDNA |
$453.00
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| 8014 | HUAEC cDNA | Human Umbilical Artery Endothelial Cell cDNA |
$453.00
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| MEDIA | |||||
| 1001 | ECM | Endothelial Cell Medium |
$139.00
As low as:
$92.00
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| 1201 | PM | Pericyte Medium |
$132.00
As low as:
$90.00
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| 1052 | ECGS | Endothelial Cell Growth Supplement |
$76.00
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| 1200 | HBVP | Human Brain Vascular Pericytes |
$790.00
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| 8000 | HUVEC | Human Umbilical Vein Endothelial Cells |
$286.00
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| 8010 | HUAEC | Human Umbilical Artery Endothelial Cells |
$477.00
|
||
| MOLECULAR BIOLOGY | |||||
| 1004 | HBMEC cDNA | Human Brain Microvascular Endothelial Cell cDNA |
$453.00
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| 1204 | HBVP cDNA | Human Brain Vascular Pericyte cDNA |
$453.00
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