ScienCell's GeneQuery™ Human Synoviocyte Cell Biology qPCR Array Kit (GQH-SYN) is designed to facilitate gene expression profiling of 88 key genes involved in human synoviocyte cell biology, including synoviocyte markers, proliferation and invasion, extracellular matrix (ECM) and synovial fluid synthesis. Genes implicated in rheumatoid arthritis and synovial sarcoma are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

• Synoviocyte markers: CD55, CD14, MS4A1, ICAM1, ITGB1, CD40, CR2, VCAM1, DRC1, CDH11, THY1, VIM
• Proliferation and invasion: NGF, TNFSF13, MIF, ESM1, EREG, IL1B, TNF, SAAL1, CDK6, POSTN, TWIST1, TRIM28, SMAD7, CCL2/5, SYVN1
• Synovial fluid synthesis: PRG4, HP, IGF1, UGDH, PTGS1/2, PTGES, HAS1, ALB, TF, IFNG, IL6
• Cell adhesion and ECM synthesis: CDH11, ITGAL, VCAM1, ITGA4, ADM, VTN, FN1, COL1A1, RAMP2/3, MMP1/8, AKT1, COMP
• Genes implicated in
• rheumatoid arthritis: LBH, PTPN22, SLC22A4, CIITA, PADI4, HLA-DRB1, CD244, IL10, SH2D1A, PREP
• synovial sarcoma: SSX1/2/3/4, SS18, SYT1, FLI1, MUC1, HMGA2, IGF1R, MDM2, CTAG1B

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.