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How can we help you today?
Need help with product selection and ordering?
Email us at sales@sciencellonline.com
Need help with scientific inquiries, technical assistance, product applications, and use?
Email us at techsupport@sciencellonline.com
Need help with issues concerning this website?
Email us at webmaster@sciencellonline.com
Have any marketing related inquiries?
Email us at marketing@sciencellonline.com
Need help ordering from abroad?
As the largest cell provider, we are available worldwide.
Please visit our Distributors page to select the company nearest to your location.
We are located at 1610 Faraday Ave, Carlsbad, CA 92008
Call us at (877) 602-8549 or (760) 602-8549
Fax us at (760) 602-8575
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Human Neural Stem Cells (Cat. #1650) from ScienCell Research Laboratories are differentiated from a human induced pluripotent stem cell line (hiPSC), which is generated using mRNA reprogramming technology from Human Dermal Fibroblasts (HDF). The monolayer HDF-hiPSC are efficiently converted to neural epithelium using HPSC Neural Induction Medium (PSCNIM, Cat. #5931), a serum-free medium for rapid and efficient neural induction of human pluripotent stem cells (hPSCs). Synergistic inhibition of glycogen synthase kinase 3 (GSK3) and transforming growth factor b (TGF-b) differentiates HDF-hiPSCs to homogenous neural stem cells (NSC) in the presence of human leukemia inhibitory factor (LIF) within 7 days.
The derived NSC are characterized by immunofluorescence with antibodies specific to nestin and SOX2. The cell population is highly pure: >90% of cells express Nestin and >80% of cells are SOX2 positive. After reviving, NSC can be maintained in HPSC Neural Induction Medium as an adherent culture or form neural spheres in EGF and bFGF containing neural stem cell medium. The cells are able to form rosette and neural tube-like structures after being passaged and plated at high density on Matrigel coated culture vessels in neural stem medium containing EGF and bFGF. HiPSC-NSC can be further expanded for months as a monolayer on MatrigelTM coated culture vessels or in suspension as neural spheres when seeded on ultra-low binding culture vessels in neural stem cell medium. NSC are multipotent and able to differentiate into various neuronal and glial subtypes. Specific patterning cues, such as SHH, retinoic acid and FGF8, can be added after reviving to direct the cells to different neural lineages.
HiPSC-NSC are cryopreserved at passage 0 and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. Cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
Catalog No. | 1650 |
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Country of Manufacture | United States |
Product Code | HiPSC-NSC |
Size/Quantity | 1 x 10^6 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Chambers SM, Fasano CA, Papapetrou EP, Tomishima M, Sadelain M, Studer L. (2009) "Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling." Nat Biotechnol. 27(3): 275-280. [2] Li W, Sun W, Zhang Y, Wei W, Ambasudhan R, Xia P, Talantova M, Lin T, Kim J, Wang X, Kim W, Lipton SA, Zhang K, Ding S. (2011) "Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors." PNAS. 108(20): 8299-8304. |
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CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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MEDIA | |||||
5931 | PSCNIM | HPSC Neural Induction Medium |
$470.00
As low as:
$168.00
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