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- Human Adipose-derived Mesenchymal Stem Cells (HMSC-ad)
Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties, in combination with their developmental plasticity, have generated tremendous interest because of the potential use of MSC in regenerative medicine to replace damaged tissues. MSC cultured without serum in the presence of transformation growth factors will differentiate into chondrocytes. In contrast, MSC cultured in serum with ascorbic acid and dexamethasone will differentiate into osteoblasts. MSC have the capability for renewal and differentiation into various lineages of mesenchymal tissues. With their renewal capability, MSC have the potential to be transplanted into an injured site or seeded on a biomimetic scaffold
to generate appropriate tissue constructs.
HMSC-ad from ScienCell Research Laboratories are isolated from human adipose tissue. HMSC-ad are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HMSC-ad are characterized by immunofluorescence with antibodies specific to CD73 and/or CD90, Oil Red O staining after adipogenic differentiation, and Alizarin Red staining after osteogenic differentiation. HMSC-ad are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMSC-ad are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. #7501) for culturing HMSC-ad in vitro.
| Catalog No. | 7510 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HMSC-ad |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | 1. Kassem, M. Mesenchymal stem cells: biological characteristics and potential clinical applications. 2004. Cloning Stem Cells. 6(4):369-74. 2. Barry, F. P., and J. M. Murphy. Mesenchymal stem cells: clinical applications and biological characterization. 2004. Int J Biochem Cell Biol. 36(4):568-84. |
75101.) Li, W., Zheng, Y., Zhao, X., Ge, Y., Chen, T., Liu, Y. & Zhou, Y. (2016) Osteoinductive Effects of Free and Immobilized Bone Forming Peptide-1 on Human Adipose-Derived Stem Cells PLoS One. 11
2.) Lee, M.S., Yuan, H., Jeon, H., Zhu, Y., Yoo, S., Shi, S., Krueger, B., Renne, R., Lu, C., Jung, J.U. & Gao, S.J. (2016) Human Mesenchymal Stem Cells of Diverse Origins Support Persistent Infection with Kaposi?s Sarcoma-Associated Herpesvirus and Manifest Distinct Angiogenic, Invasive, and Transforming Phenotypes mBio. 7
3.) Wang, X.F., Song, Y., Liu, Y.S., Sun, Y., Wang, Y., Wang, Y. & Lyu, P.J.(2016) 'Osteogenic Differentiation of ThreeDimensional Bioprinted Constructs Consisting of Human AdiposeDerived Stem Cells In Vitro and In Vivo' PLoS One. VOL 11
4.) Lee HK, Finniss S, Cazacu S, Bucris E, Ziv-Av A, Xiang C, Bobbitt K, Rempel SA, Hasselbach L, Mikkelsen T, Slavin S, Brodie C. (2013) "Mesenchymal stem cells deliver synthetic microRNA mimics to glioma cells and glioma stem cells and inhibit their cell migration and self-renewal." Oncotarget. 4: 346-61.
5.) Matsuse D, Kitada M, Kohama M, Nishikawa K, Makinoshima H, Wakao S, Fujiyoshi Y, Heike T, Nakahata T, Akutsu H, Umezawa A, Harigae H, Kira J, Dezawa M. (2010) "Human umbilical cord-derived mesenchymal stromal cells differentiate into functional Schwann cells that sustain peripheral nerve regeneration." J Neuropathol Exp Neurol. 69: 973-85.
6.) Schoolmeesters A, Eklund T, Leake D, Vermeulen A, Smith Q, Force Aldred S, Fedorov Y. (2009) "Functional profiling reveals critical role for miRNA in differentiation of human mesenchymal stem cells." PLoS One. 4: e5605.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 7140 | HAMSC | Human Amniotic Mesenchymal Stromal Cells |
$738.00
|
||
| 7150 | HCMSC | Human Chorionic Mesenchymal Stromal Cells |
$738.00
|
||
| 7500 | HMSC-bm | Human Bone Marrow-derived Mesenchymal Stem Cells |
$738.00
|
||
| 7520 | HMSC-he | Human Liver-derived Mesenchymal Stem Cells |
$738.00
|
||
| 7530 | HUMSC | Human Umbilical Mesenchymal Stem Cells |
$738.00
|
||
| MEDIA | |||||
| 7501 | MSCM | Mesenchymal Stem Cell Medium |
$126.00
As low as:
$81.00
|
||
| 7511 | MSCM-sf | Mesenchymal Stem Cell Medium-serum free |
$208.00
As low as:
$104.00
|
||
| 7521 | MSCM-acf | Mesenchymal Stem Cell Medium-animal component free |
$232.00
As low as:
$115.00
|
||
| 7551 | MCDM | Mesenchymal Stem Cell Chondrogenic Differentiation Medium |
$284.00
As low as:
$104.00
|
||
| 7531 | MODM | Mesenchymal Stem Cell Osteogenic Differentiation Medium |
$336.00
As low as:
$104.00
|
||
| 7541 | MADM | Mesenchymal Stem Cell Adipogenic Differentiation Medium |
$349.00
|
||
| 7540 | HPMSC | Human Pulmonary Mesenchymal Stem Cells |
$738.00
|
||
| REAGENTS | |||||
| 0843 | ORed | Oil Red O Staining Kit |
$100.00
|
||
| 8298 | HMSC-A-qPCR | Human Mesenchymal Stem Cell Adipogenesis Detection qPCR Kit |
$282.00
|
||
| 0223 | ARed | Alizarin Red S Staining Kit |
$78.00
|
||
| 8328 | HMSC-C-qPCR | Human Mesenchymal Stem Cell Chondrogenesis Detection qPCR Kit |
$282.00
|
||
| 8348 | SafraninO | Safranin O Staining Kit |
$106.00
|
||
| 8378 | ABlue | Alcian Blue Staining Kit |
$106.00
|
||
| 8678 | ARed-Q | Alizarin Red S Staining Quantification Assay |
$170.00
|
||
| 0933 | MesenF | MesenFectagen |
$253.00
|
||
| MOLECULAR BIOLOGY | |||||
| 7514 | HMSC-ad cDNA | Human Mesenchymal Stem Cell-adipose cDNA |
$411.00
|
||
| 7515 | HMSC-ad tRNA | Human Mesenchymal Stem Cell-adipose Total RNA |
$364.00
|
||
| 7516 | HMSC-ad Lysate | Human Mesenchymal Stem Cell-adipose Lysate |
$398.00
|
||
| 7517 | HMSC-ad miRNA | Human Mesenchymal Stem Cell-adipose MicroRNA |
$340.00
|
||
| 7519 | HMSC-a gDNA | Human Mesenchymal Stem Cell-adipose Genomic DNA |
$398.00
|
||
