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- Human Adipose Microvascular Endothelial Cells
Microvascular endothelial cells line blood vessels and contribute to many biological processes such as angiogenesis, coagulation, trafficking of lymphocytes, and the inflammatory response. Microvascular endothelial cells are diverse and have specific cellular characteristics and functions depending on the organ/tissue in which they are located. Adipose tissue is unique because it has the capacity to continually grow throughout adult life. Thus, it has a high level of angiogenesis to provide the extensive vascularization required for adipose tissue. Studies have shown that angiogenesis precedes adipogenesis, implying that microvascular endothelial cells influence the proliferation of preadipocytes. At the same time, microvascular endothelial cell growth is stimulated by adipocyte secreted VEGG, suggesting a complex paracrine relationship between microvascular endothelial cells and preadipocytes during tissue development.
HAMEC from ScienCell Research Laboratories are isolated from human adipose tissue. HAMEC are cryopreserved and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HAMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM) and by uptake of DiI-Ac-LDL. HAMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for the culturing of HAMEC.
Catalog No. | 7200 |
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Country of Manufacture | United States |
Product Code | HAMEC |
Size/Quantity | 5 x 10^5 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Crandall D.L., Hausman G.J., Kral J.G. (1997) A review of the microcirculation of adipose tissue: anatomic, metabolic, and angiogenic perspectives. Microcirculation 4(2):211-32 [2] Hutley, L. J., Herington, A. C., Shurety, W., Cheung, C., Vesey, D. A., Cameron, D. P., Prins, J. B. (2000) Human adipose tissue endothelial cells promote preadipocyte proliferation. American Journal of Physiology: Endocrinology and Metabolism. 281(5):E1037-E1044 [3] Hausman, G. J., Richardson, R. L. (2004) Adipose tissue angiogenesis. Journal of Animal Science. 82(3): 925-934 |
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CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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MEDIA | |||||
1001 | ECM | Endothelial Cell Medium |
$126.00
As low as:
$83.00
|
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REAGENTS | |||||
0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
|
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0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
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0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
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0903 | EndoF | EndoFectagen |
$253.00
|
||
8248 | BPF | Bovine Plasma Fibronectin |
$124.00
|
||
MOLECULAR BIOLOGY | |||||
7204 | HAMEC cDNA | Human Adipose Microvascular Endothelial Cell cDNA |
$411.00
|
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7205 | HAMEC tRNA | Human Adipose Microvascular Endothelial Cell Total RNA |
$364.00
|
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7206 | HAMEC Lysate | Human Adipose Microvascular Endothelial Cell Lysate |
$398.00
|
||
7207 | HAMEC miRNA | Human Adipose Microvascular Endothelial Cell MicroRNA |
$340.00
|
||
7209 | HAMEC gDNA | Human Adipose Microvascular Endothelial Cell Genomic DNA |
$398.00
|