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- Human Adipose Microvascular Endothelial Cells
Microvascular endothelial cells line blood vessels and contribute to many biological processes such as angiogenesis, coagulation, trafficking of lymphocytes, and the inflammatory response. Microvascular endothelial cells are diverse and have specific cellular characteristics and functions depending on the organ/tissue in which they are located. Adipose tissue is unique because it has the capacity to continually grow throughout adult life. Thus, it has a high level of angiogenesis to provide the extensive vascularization required for adipose tissue. Studies have shown that angiogenesis precedes adipogenesis, implying that microvascular endothelial cells influence the proliferation of preadipocytes. At the same time, microvascular endothelial cell growth is stimulated by adipocyte secreted VEGG, suggesting a complex paracrine relationship between microvascular endothelial cells and preadipocytes during tissue development.
HAMEC from ScienCell Research Laboratories are isolated from human adipose tissue. HAMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HAMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HAMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for the culturing of HAMEC.
| Catalog No. | 7200 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HAMEC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Crandall D.L., Hausman G.J., Kral J.G. (1997) A review of the microcirculation of adipose tissue: anatomic, metabolic, and angiogenic perspectives. Microcirculation 4(2):211-32 [2] Hutley, L. J., Herington, A. C., Shurety, W., Cheung, C., Vesey, D. A., Cameron, D. P., Prins, J. B. (2000) Human adipose tissue endothelial cells promote preadipocyte proliferation. American Journal of Physiology: Endocrinology and Metabolism. 281(5):E1037-E1044 [3] Hausman, G. J., Richardson, R. L. (2004) Adipose tissue angiogenesis. Journal of Animal Science. 82(3): 925-934 |
72001.) Yang, Q. et al. Disruption of endothelial Pfkfb3 ameliorates diet-induced murine insulin resistance. Journal of Endocrinology 250, 93–104 (2021).
2.) Pettinato, G. et al. Spectroscopic label-free microscopy of changes in live cell chromatin and biochemical composition in transplantable organoids. Science Advances 7, (2021).
3.) Mylvaganam, S., Riedl, M., Vega, A., Collins, R. F., Jaqaman, K., Grinstein, S., & Freeman, S. A. (2020). Stabilization of endothelial receptor arrays by a polarized spectrin cytoskeleton facilitates rolling adhesion of Leukocytes. SSRN Electronic Journal. https://doi.org/10.2139/ssrn.3541188
4.) Haynes, B. A., Yang, L. F., Huyck, R. W., Lehrer, E. J., Turner, J. M., Barabutis, N., … Dobrian, A. D. (2019). Endothelial-to-Mesenchymal Transition in Human Adipose Tissue Vasculature Alters the Particulate Secretome and Induces Endothelial Dysfunction. Arteriosclerosis, Thrombosis, and Vascular Biology. doi:10.1161/atvbaha.119.31282
5.) Pettinato, G. et al. Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with endothelial cells. Scientific Reports 9, (2019).
6.) Marques, P. E., Nyegaard, S., Collins, R. F., Troise, F., Freeman, S. A., Trimble, W. S., & Grinstein, S. (2019). Multimerization and retention of the scavenger receptor SR-B1 in the plasma membrane. Developmental Cell, 50(3). https://doi.org/10.1016/j.devcel.2019.05.026
7.) Hilfenhaus, G. et al. Vav3-induced cytoskeletal dynamics contribute to heterotypic properties of endothelial barriers. Journal of Cell Biology 217, 2813–2830 (2018).
8.) Haynes, B. A., Huyck, R. W., James, A. J., Carter, M. E., Gaafar, O. U., Day, M., Pinto, A., & Dobrian, A. D. (2018). Isolation, expansion, and adipogenic induction of CD34+CD31+ endothelial cells from human omental and subcutaneous adipose tissue. Journal of Visualized Experiments, (137). https://doi.org/10.3791/57804
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1001 | ECM | Endothelial Cell Medium |
$126.00
As low as:
$83.00
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| REAGENTS | |||||
| 0103 | T/E | Trypsin/EDTA Solution (0.25%; 100ml) |
$21.00
|
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
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| 0903 | EndoF | EndoFectagen |
$253.00
|
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| 8248 | BPF | Bovine Plasma Fibronectin |
$124.00
|
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| MOLECULAR BIOLOGY | |||||
| 7204 | HAMEC cDNA | Human Adipose Microvascular Endothelial Cell cDNA |
$411.00
|
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| 7205 | HAMEC tRNA | Human Adipose Microvascular Endothelial Cell Total RNA |
$364.00
|
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| 7206 | HAMEC Lysate | Human Adipose Microvascular Endothelial Cell Lysate |
$398.00
|
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| 7207 | HAMEC miRNA | Human Adipose Microvascular Endothelial Cell MicroRNA |
$340.00
|
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| 7209 | HAMEC gDNA | Human Adipose Microvascular Endothelial Cell Genomic DNA |
$398.00
|
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