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- Human Aortic Endothelial Cells
The aorta, the largest artery in the human body, distributes oxygenated blood from the heart to all parts of the body. Lining the vessel wall, aortic endothelial cells (AEC) are constantly exposed to high hemodynamic forces that induce changes in the production of endothelium-derived substances regulating vasoconstriction and vessel growth. AEC also modulate the expression of cellular adhesion molecules to control and fine-tune inflammatory responses and fibrinolysis. These physiological properties allow AEC cultures to be widely used in the study of mechanisms for endothelium dysfunction, pathogenesis of vascular diseases and atherosclerosis, and the development of novel disease treatments.
HAEC from ScienCell Research Laboratories are isolated from human aorta. HAEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HAEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM). HAEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HAEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HAEC in vitro.
| Catalog No. | 6100 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HAEC |
| Size/Quantity | 5 x 10⁵ cells/vial. |
| Product Use | HAEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Elhadj, S. and Forsten, K. E. (2001) Chronic shear stress affects bovine aortic endothelial cell secreted proteoglycan production in vitro. Bioengineering Conference 50:767-768. [2] Donnini, D., Perrella, G., Stel, G., Ambesi-Impiombato, F. S., Curcio, F. (2000) A new model of human aortic endothelial cells in vitro. Biochimie 82:1107-14. |
61001.) Zhu, D.D., Tang, R.N., Lv, L.L., Wen, Y., Liu, H., Zhang, X.L., Ma, K.L. & Liu, B.C. (2016) Interleukin-1? mediates high glucose induced phenotypic transition in human aortic endothelial cells Cardiovasc Diabetol. 15
2.) Zhao, Y., Zhang, X., Li, J., Bian, Y., Sheng, M., Liu, B., Fu, Z., Zhang, Y. & Yang, B. (2016) Jujuboside B Reduces Vascular Tension by Increasing Ca2+ Influx and Activating Endothelial Nitric Oxide Synthase PLoS One. 11
3.) Zhang, Y., Wu, X., Li, Y., Zhang, H., Li, Z., Zhang, Y., Zhang, L., Ju, J., Liu, X., Chen, X., Glybochko, P.V., Nikolenko, V., Kopylov, P., Xu, C. & Yang, B.(2016) 'Endothelial to mesenchymal transition contributes to arsenictrioxideinduced cardiac fibrosis' Sci Rep. VOL 6
4.) Wu, W., Xu, H., Wang, Z., Mao, Y., Yuan, L., Luo, W., Cui, Z., Cui, T., Wang, X.L. & Shen, Y.H. (2015) PINK1-Parkin-Mediated Mitophagy Protects Mitochondrial Integrity and Prevents Metabolic Stress-Induced Endothelial Injury PLoS One. 10
5.) Pahl, M.C., Erdman, R., Kuivaniemi, H., Lillvis, J.H., Elmore, J.R. & Tromp, G. (2015) Transcriptional (ChIP-Chip) Analysis of ELF1, ETS2, RUNX1 and STAT5 in Human Abdominal Aortic Aneurysm Int J Mol Sci. 16
6.) Lin, S., Nadeau, P.E. & Mergia, A. (2015) HIV inhibits endothelial reverse cholesterol transport through impacting subcellular Caveolin-1 trafficking Retrovirology. 12
7.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
8.) Zhao N, Watson N, Xu Z, Chen Y, Waterman J, Sankar J, Zhu D. (2014) "In Vitro Biocompatibility and Endothelialization of Novel Magnesium-Rare Earth Alloys for Improved Stent Applications." PloS one. 9: e98674.
9.) Wang W, Deng M, Liu X, Ai W, Tang Q, Hu J. (2011) "TLR4 Activation Induces Nontolerant Inflammatory Response in Endothelial Cells." Inflammation. 34: 509-18.
10.) Cai W, Torreggiani M, Zhu L, Chen X, He JC, Striker GE, Vlassara H. (2010) "AGER1 regulates endothelial cell NADPH oxidase-dependent oxidant stress via PKC-delta: implications for vascular disease." Am J Physiol Cell Physiol. 298: C624-34.
11.) Kong DK, Georgescu SP, Cano C, Aronovitz MJ, Iovanna JL, Patten RD, Kyriakis JM, Goruppi S. (2010) "Deficiency of the transcriptional regulator p8 results in increased autophagy and apoptosis, and causes impaired heart function." Mol Biol Cell. 21: 1335-49.
12.) Shahani T, Lavend'homme R, Luttun A, Saint-Remy JM, Peerlinck K, Jacquemin M. (2010) "Activation of human endothelial cells from specific vascular beds induces the release of a FVIII storage pool." Blood. 115: 4902-9.
13.) Zhang CL, Song F, Zhang J, Song QH. (2010) "Hypoxia-induced Bcl-2 expression in endothelial cells via p38 MAPK pathway." Biochem Biophys Res Commun. 394: 976-80.
14.) Linden E, Cai W, He JC, Xue C, Li Z, Winston J, Vlassara H, Uribarri J. (2008) "Endothelial dysfunction in patients with chronic kidney disease results from advanced glycation end products (AGE)-mediated inhibition of endothelial nitric oxide synthase through RAGE activation." Clin J Am Soc Nephrol. 3: 691-8.
15.) Zhang X, Baughman CB, Kaplan DL. (2008) "In vitro evaluation of electrospun silk fibroin scaffolds for vascular cell growth." Biomaterials. 29: 2217-27.
16.) Gentry M, Taormina J, Pyles RB, Yeager L, Kirtley M, Popov VL, Klimpel G, Eaves-Pyles T. (2007) "Role of Primary Human Alveolar Epithelial Cells in Host Defense against Francisella tularensis Infection." Infect Immun. 75: 3969-78.
17.) Simard JM, Tsymbalyuk O, Ivanov A, Ivanova S, Bhatta S, Geng Z, Woo SK, Gerzanich V. (2007) "Endothelial sulfonylurea receptor 1-regulated NCCa-ATP channels mediate progressive hemorrhagic necrosis following spinal cord injury." J Clin Invest. 117: 2105-13.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1001 | ECM | Endothelial Cell Medium |
$126.00
As low as:
$83.00
|
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| REAGENTS | |||||
| 0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
|
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
||
| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
||
| 0903 | EndoF | EndoFectagen |
$253.00
|
||
| 8248 | BPF | Bovine Plasma Fibronectin |
$124.00
|
||
| MOLECULAR BIOLOGY | |||||
| 6104 | HAEC cDNA | Human Aortic Endothelial Cell cDNA |
$411.00
|
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| 6105 | HAEC tRNA | Human Aortic Endothelial Cell Total RNA |
$364.00
|
||
| 6106 | HAEC Lysate | Human Aortic Endothelial Cell Lysate |
$398.00
|
||
| 6107 | HAEC miRNA | Human Aortic Endothelial Cell MicroRNA |
$340.00
|
||
| 6109 | HAEC gDNA | Human Aortic Endothelial Cell genomic DNA |
$398.00
|
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