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- Human Cardiac Myocytes-adult
Cardiac myocytes are the most physically energetic cells in the body. They are highly specialized high-oxygen-content cells that house a large number of mitochondria [1]. They occupy as much as 75% of the cardiac mass, but constitute only about one third of the total cell number in the heart. Differentiated cardiac myocytes have little capacity to proliferate; however, hypertrophic growth has been shown to respond to alpha1-adrenergic stimuli via the Ras/MEK pathway [2]. All cardiac myocytes are capable of spontaneous rhythmic depolarization and repolarization of their membranes. Contraction of cardiac myocytes is myogenic, which is independent of nervous stimulation. There is a complex network of signals in cardiac myocytes regulating the rhythmic pumping of the heart [3]. Cardiac myocyte hypertrophy and apoptosis have been implicated in the loss of contractile function during heart failure. A better understanding of the cardiac signaling network will help reveal the cellular mechanisms leading to cardiac myocyte death.
HCM-a from ScienCell Research Laboratories are isolated from human adult heart. HCM-a are cryopreserved after purification and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HCM-a are characterized by immunofluorescence with antibody specific to sarcomeric α-actinin. HCM-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCM-a are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HCM-a are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.
Recommended Medium
It is recommended to use Cardiac Myocyte Medium-serum free (CMM-sf, Cat. #6101) for culturing HCM-a in vitro.
Catalog No. | 6210 |
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Country of Manufacture | United States |
Product Code | HCM-a |
Size/Quantity | 1 x 10^6 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Bodyak, N., Kang, P. M., Hiromura, M., Sulijoadikusumo, I., Horikoshi, N., Khrapko, K. and Usheva, A. (2002) Gene expression profiling of the aging mouse cardiac myocytes. Nucleic Acids Research 30(17):3788-3794. [2] Tamamori-Adachi, M., Ito, H., Nobori, K., Hayashida, K., Kawauchi, J., Adachi, S., Ikeda, M. A. and Kitajima, S. (2002) Expression of cyclin D1 and CDK4 causes hypertrophic growth of cardiomyocytes in culture: a possible implication for cardiac hypertrophy. Biochem Biophys Res Commun 296(2):274-80. [3] Sambrano, G.R., Fraser, I., Han, H., Ni, Y., O'Connell, T., Yan, Z. and Stull, J. T. (2002) Navigating the signaling network in mouse cardiac myocytes. Nature 420(6916):712-4. |
1.) d'Avenia, M., Citro, R., De Marco, M., Veronese, A., Rosati, A., Visone, R., Leptidis, S., Philippen, L., Vitale, G., Cavallo, A., Silverio, A., Prota, C., Gravina, P., De Cola, A., Carletti, E., Coppola, G., Gallo, S., Provenza, G., Bossone, E., Piscione, F., Hahne, M., De Windt, L.J., Turco, M.C. & De Laurenzi, V. (2015) A novel miR-371a-5p-mediated pathway, leading to BAG3 upregulation in cardiomyocytes in response to epinephrine, is lost in Takotsubo cardiomyopathy Cell Death Dis. 6
2.) Beckett CS, McHowat J. (2008) "Calcium-independent phospholipase A2 in rabbit ventricular myocytes." Lipids. 43: 775-82.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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MEDIA | |||||
6201 | CMM | Cardiac Myocyte Medium |
$137.00
As low as:
$90.00
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6101 | CMM-sf | Cardiac Myocyte Medium-serum free |
$137.00
As low as:
$90.00
|
||
MOLECULAR BIOLOGY | |||||
6214 | HCM-a cDNA | Human Cardiac Myocyte-adult cDNA |
$453.00
|
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6215 | HCM-a tRNA | Human Cardiac Myocyte-adult Total RNA |
$401.00
|
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6216 | HCM-a Lysate | Human Cardiac Myocyte-adult Lysate |
$438.00
|
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6217 | HCM-a miRNA | Human Cardiac Myocyte-adult MicroRNA |
$374.00
|
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6219 | HCM-a gDNA | Human Cardiac Myocyte-adult genomic DNA |
$438.00
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