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- Human Corneal Epithelial Cells
The cornea is a unique tissue due to its transparency and avascularity. The cornea consists of three distinct cell layers: the outer epithelium, the central stroma, and the inner endothelium. The corneal epithelium plays a role in the innate immune response by sensing the presence of pathogens and providing signals to activate the corneal defense system. Corneal epithelial cells also express a high level of aldehyde dehydrogenase to protect them against UV- and 4-hydroxynonenal-induced cellular damage. The renewal of corneal epithelium is regulated by a host of cytokines, including epidermal growth factor, which activates their cognate receptors in the deeper layers of the epithelium. Cultured corneal epithelial cells can be an alternative to animal testing in toxicology studies. They can also be used to understand the highly integrated balance between corneal epithelial proliferation, differentiation, and cell death.
HCEpiC from ScienCell Research Laboratories are isolated from human cornea. HCEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCEpiC are characterized by their cobble stone morphology in serum-free culture and immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HCEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCEpiC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Corneal Epithelial Cell Medium (CEpiCM, Cat. #6511) for culturing HCEpiC in vitro.
| Catalog No. | 6510 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HCEpiC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Zhang, J., Wu, X. Y. and Yu, F. S. (2005) Inflammatory response of corneal epithelial cells to pseudomonas aeruginosa infection. Curr. Eye Res. 30:527-534. [2] Pappa, A., Brown, D., Koutalos, Y., Degregori, J., White, C. and Vasiliou, V. (2005) Human aldehyde dehydrogenase 3A1 inhibits proliferation and promotes survival of human corneal epithelial cells. J. Biol. Chem. 280:27998-28006. [3]. Yang, H., Mergler, S., Sun, X., Wang, Z, Lu, L., Bonanno, J. A., Pleyer, U. and Reinach, P. S. (2005). TRPC4 knockdown suppresses EGF-induced store operated channel activation and growth in human corneal epithelial cells. J Biol Chem. |
65101.) Vellonen KS, H?kli M, Merezhinskaya N, Tervo T, Honkakoski P, Urtti A. (2010) "Monocarboxylate transport in human corneal epithelium and cell lines." Eur J Pharm Sci. 39: 241-7.
2.) Vellonen KS, Mannermaa E, Turner H, H?kli M, Wolosin JM, Tervo T, Honkakoski P, Urtti A. (2010) "Effluxing ABC transporters in human corneal epithelium." J Pharm Sci. 99: 1087-98.
3.) Belser JA, Wadford DA, Xu J, Katz JM, Tumpey TM. (2009) "Ocular infection of mice with influenza A (H7) viruses: a site of primary replication and spread to the respiratory tract." J Virol. 283: 7075-84.
4.) Cheong HI, Johnson J, Cormier M, Hosseini K. (2008) "In vitro cytotoxicity of eight beta-blockers in human corneal epithelial and retinal pigment epithelial cell lines: comparison with epidermal keratinocytes and dermal fibroblasts." Toxicol In Vitro. 22: 1070-6.
5.) Reichl S. (2008) "Cell culture models of the human cornea - a comparative evaluation of their usefulness to determine ocular drug absorption in-vitro." J Pharm Pharmacol. 60: 299-307.
6.) Zhang JZ, Cavet ME, Ward KW. (2008) "Anti-inflammatory effects of besifloxacin, a novel fluoroquinolone, in primary human corneal epithelial cells." Curr Eye Res. 33: 923-32.
7.) Hunt DW, Boivin WA, Fairley LA, Jovanovic MM, King DE, Salmon RA, Utting OB. (2006) "Ultraviolet B light stimulates interleukin-20 expression by human epithelial keratinocytes." Photochem Photobiol. 82: 1292-300.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 6511 | CEpiCM | Corneal Epithelial Cell Medium |
$135.00
As low as:
$90.00
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| MOLECULAR BIOLOGY | |||||
| 6514 | HCEpiC cDNA | Human Corneal Epithelial Cell cDNA |
$453.00
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| 6515 | HCEpiC tRNA | Human Corneal Epithelial Cell Total RNA |
$401.00
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| 6516 | HCEpiC Lysate | Human Corneal Epithelial Cell Lysate |
$438.00
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| 6517 | HCEpiC miRNA | Human Corneal Epithelial Cell MicroRNA |
$374.00
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| 6519 | HCEpiC gDNA | Human Corneal Epithelial Cell Genomic DNA |
$438.00
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