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- Human Dermal Fibroblasts-neonatal
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in various organs are intrinsically different. Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. In addition, they secrete large quantities of hyaluronan in response to inflammatory stimuli.
HDF-n from ScienCell Research Laboratories are isolated from neonatal human skin. HDF-n are cryopreserved at passage one and delivered frozen. Each vial contains > 5 x 10^5 cells in 1 ml volume. HDF-n are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HDF-n are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HDF-n are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HDF-n in vitro.
| Catalog No. | 2310 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HDF-n |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137. [2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981. [3] Stair S, Carlson KW, Shuster S, Wei ET, Stern R (2002) Mystixin peptides reduce hyaluronan deposition and edema formation. Eur J Pharmacol 30;450(3):291-6. |
23101.) Frandsen, S.K., Gibot, L., Madi, M., Gehl, J. & Rols, M.P. (2015) Calcium Electroporation: Evidence for Differential Effects in Normal and Malignant Cell Lines, Evaluated in a 3D Spheroid Model PLoS One. 10
2.) Dang Y, Ye X, Weng Y, Tong Z, Ren Q. (2010) "Effects of the 532-nm and 1,064-nm Q-switched Nd:YAG lasers on collagen turnover of cultured human skin fibroblasts: a comparative study." Lasers Med Sci. 25: 719-26.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| REAGENTS | |||||
| 0103 | T/E | Trypsin/EDTA Solution (0.25%; 100ml) |
$21.00
|
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
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| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
|
||
| 0913 | FibroF | FibroFectagen |
$253.00
|
||
| MOLECULAR BIOLOGY | |||||
| 2314 | HDF-n cDNA | Human Dermal Fibroblast-neonate cDNA |
$411.00
|
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| 2315 | HDF-n tRNA | Human Dermal Fibroblast-neonatal Total RNA |
$364.00
|
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| 2316 | HDF-n Lysate | Human Dermal Fibroblast-neonate Lysate |
$398.00
|
||
| 2317 | HDF-n miRNA | Human Dermal Fibroblast-neonate MicroRNA |
$340.00
|
||
| 2319 | HDF-n gDNA | Human Dermal Fibroblast-neonate genomic DNA |
$398.00
|
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