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- Human Epidermal Melanocytes-light
Melanocytes are neural crest-derived cells that produce melanin via melanogenesis. Melanocytes localize to several tissues including the epidermis, eye, inner ear and leptomeninges. Dysregulation of melanocyte migration, proliferation, or survival during embryonic development thus causes congenital disorders in those tissues as seen in Tietz syndrome, Waardenburg syndrome, and piebaldism. In the bottom layer of skin epidermis, melanocytes synthesize and transfer dark-colored melanin to surrounding keratinocytes to give skin pigmentation. Melanin also blocks UV-B light to protect the hypodermis from solar exposure-induced photodamage. Progress in culture techniques, along with an improved understanding of melanocyte biology, has led to a successful culture system to model melanomas, inner ear homeostasis, vitiligo, and
mitochondrial dysfunction in Duchenne Muscular Dystrophy.
HEM-l from ScienCell Research Laboratories is isolated from neonatal human skin. HEM-l are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HEM-l are characterized by immunofluorescence with antibodies specific to S100β and/or NGF-receptor (p75). HEM-l are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HEM-l are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Melanocyte Medium (MelM, Cat. #2201) for culturing HEM-l in vitro.
| Catalog No. | 2200 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HEM-l |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1]. Eisinger, M. and Marko, O. (1982) Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin. Proc. Natl. Acad. USA 79:2018-2022. [2]. Tang, A., Eller, M. S., Hara, M., Yaar, M., Hirohashi, S. and Gilchrest, B. A. (1994) E-cadherin is the major mediator of human melanocyte adhesion to keratinocytes in vitro. J. Cell Sci. 107:983-992. [3]. Shioda, T., Fenner, M. H. and Isselbacher, K. J. (1996) msg1, a novel melanocyte-specific gene, encodes a nuclear protein and is associated with pigmentation. Proc. Natl. Acad. Sci. USA 93:12298-12303. |
22001.) Adini I, Ghosh K, Adini A, Chi ZL, Yoshimura T, Benny O, Connor KM, Rogers MS, Bazinet L, Birsner AE, Bielenberg DR, D'Amato RJ."Melanocyte-secreted fibromodulin promotes an angiogenic microenvironment." 2014 Jan;124(1):425-36. doi: 10.1172/JCI69404. Epub 2013 Dec 20.
2.) Yang J, Bill MA, Young GS, La Perle K, Landesman Y, Shacham S, Kauffman M, Senapedis W, Kashyap T, Saint-Martin JR, Kendra K, Lesinski GB. (2014) 'Novel Small Molecule XPO1/CRM1 Inhibitors Induce Nuclear Accumulation of TP53, Phosphorylated MAPK and Apoptosis in Human Melanoma Cells.'
3.) Yang J, Bill MA, Young GS, La Perle K, Landesman Y, Shacham S, Kauffman M, Senapedis W, Kashyap T, Saint-Martin JR, Kendra K, Lesinski GB. (2014) "Novel Small Molecule XPO1/CRM1 Inhibitors Induce Nuclear Accumulation of TP53, Phosphorylated MAPK and Apoptosis in Human Melanoma Cells." PloS one. 9: e102983.
4.) Li JL, Mazar J, Zhong C, Faulkner GJ, Govindarajan SS, Zhang Z,Dinger ME, Meredith G, Adams C, Zhang S, Mattick JS, Ray A, Perera RJ (2013) 'Genome-wide methylated CpG island profiles of melanoma cells reveal a melanoma coregulation network.'
5.) Li JL, Mazar J, Zhong C, Faulkner GJ, Govindarajan SS, Zhang Z,Dinger ME, Meredith G, Adams C, Zhang S, Mattick JS, Ray A, Perera RJ (2013) "Genome-wide methylated CpG island profiles of melanoma cells reveal a melanoma coregulation network." . 3.
6.) Mazar J, DeYoung K, Khaitan D, Meister E, Almodovar A, Goydos J, Ray A, Perera RJ. (2010) "The regulation of miRNA-211 expression and its role in melanoma cell invasiveness." PLoS One. 5: e13779.
7.) Savaraj N, You M, Wu C, Wangpaichitr M, Kuo MT, Feun LG. (2010) "Arginine deprivation, autophagy, apoptosis (AAA) for the treatment of melanoma." Curr Mol Med. 10: 405-12.
8.) Doudican N, Rodriguez A, Osman I, Orlow SJ. (2008) "Mebendazole induces apoptosis via Bcl-2 inactivation in chemoresistant melanoma cells." Mol Cancer Res. 6: 1308-15.
9.) Palmer MB, Majumder P, Green MR, Wade PA, Boss JM. (2007) "A 3' Enhancer Controls Snail Expression in Melanoma Cells." Cancer Res. 67: 6113-20.
10.) May KM, Vogt A, Bachas LG, Anderson KW. (2005) "Vascular endothelial growth factor as a biomarker for the early detection of cancer using a whole cell-based biosensor." Anal Bioanal Chem. 382: 1010-6.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 2201 | MelM | Melanocyte Medium |
$130.00
As low as:
$77.00
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| MOLECULAR BIOLOGY | |||||
| 2204 | HEM cDNA | Human Epidermal Melanocyte cDNA |
$453.00
|
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| 2205 | HEM tRNA | Human Epidermal Melanocyte-light Total RNA |
$401.00
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| 2206 | HEM-l Lysate | Human Epidermal Melanocyte-light Lysate |
$438.00
|
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| 2207 | HEM miRNA | Human Epidermal Melanocyte MicroRNA |
$374.00
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| 2209 | HEM-l gDNA | Human Epidermal Melanocyte genomic DNA |
$438.00
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