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The mammalian lens consists of two cell types, lens fiber cells which form the bulk of the lens, and a monolayer of epithelial cells that cover the anterior surface of the fibers. Lens epithelial cells are responsible for homeostasis regulation of the lens, including electrolyte and fluid transport [1]. Under normal development, lens epithelial cells progressively differentiate and mature. Lens epithelial cells then migrate from the equatorial region into the interior of the lens to produce transparent crystallins, elongate to form lens fiber cells, and eventually lose their nuclei and other organelles [2]. Studies have shown that lens epithelial cell differentiation and lens polarization are regulated by growth factors present in the ocular fluids [3], such as epidermal growth factor, basic fibroblast growth factor, insulin growth factor, and insulin [4].
HLEpiC from ScienCell Research Laboratories are isolated from human lens. HLEpiC are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HLEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18, -19, and fibronectin. HLEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HLEpiC are guaranteed to further expand for 10 population doublings under the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Epithelial Cell Medium (EpiCM, Cat. #4101) for culturing HLEpiC in vitro.
Catalog No. | 6550 |
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Country of Manufacture | United States |
Product Code | HLEpiC |
Size/Quantity | 5 x 10^5 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
Shipping Info | Dry ice. |
References | [1]. Wagner, L. M. and Takemoto, D. J. (2001) PKCa and PKC overexpression causes lentoid body formation in the N/N 1003A rabbit lens epithelial cell line. Molecular Vision 7: 138-144. [2]. Lang, R. A. (1999) Which factors stimulate lens fiber cell differentiation in vivo Invest Opthalmol Vis Sci 40:3075-8. [3]. Leenders, W. P., van Genesen, S. T., Schoenmakers, J. G., van Zoelen, E. J., Lubsen, N. H. (1997) Synergism between temporally distinct growth factors: bFGF, insulin and lens cell differentiation. Mech Dev. 67:193-201. |
1.) Michael I, Shmoish M, Walton DS, Levenberg S. (2008) "Interactions between trabecular meshwork cells and lens epithelial cells: a possible mechanism in infantile aphakic glaucoma." Invest Ophthalmol Vis Sci. 49: 3981-7.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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MEDIA | |||||
4101 | EpiCM | Epithelial Cell Medium |
$122.00
As low as:
$81.00
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REAGENTS | |||||
0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
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0103 | T/E | Trypsin/EDTA Solution (0.25%; 100ml) |
$21.00
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0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
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0923 | EpiF | EpiFectagen |
$253.00
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MOLECULAR BIOLOGY | |||||
6554 | HLEpiC cDNA | Human Lens Epithelial Cell cDNA |
$411.00
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6555 | HLEpiC tRNA | Human Lens Epithelial Cell Total RNA |
$364.00
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6556 | HLEpiC Lysate | Human Lens Epithelial Cell Lysate |
$398.00
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6557 | HLEpiC miRNA | Human Lens Epithelial Cell MicroRNA |
$340.00
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6559 | HLEpiC gDNA | Human Lens Epithelial Cell Genomic DNA |
$398.00
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