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- Human Lymphatic Endothelial Cells
The lymphatic system is an essential part of the immune system. It helps maintain tissue homeostasis, including interstitial protein transport, tissue fluid balance, and development of cellular immunity. Lymph nodes, which are located throughout the lymphatic system, contain large numbers of lymphocytes, macrophages and antigen presenting cells that together initiate primary immune response. Specialized lymphatic endothelial cells (LEC) in the cortex assist in the primary immune response by recruiting intravascular lymphocytes as they circulate past. In addition, LEC are responsible for facilitating the transmigration of intravascular lymphocytes into the reticular meshwork where the lymphocytes can interact with antigen presenting cells. LEC have been shown to be associated with chronic inflammation and cancers.
HLEC from ScienCell Research Laboratories are isolated from human lymph node. HLEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HLEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HLEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HLEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HLEC in vitro.
| Catalog No. | 2500 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HLEC |
| Size/Quantity | 5 x 10⁵ cells/vial. |
| Product Use | HLEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Swartz, A. and Skobe, M. (2001) Lymphatic function, lymphangiogenesis, and cancer metastasis. Microsc. Res. Tech. 55:92-99. [2] Leak, L. V. (1970) Electron microscopic observations on lymphatic capillaries and the structural components of the connective tissue-lymph interface. Microvasc. Res. 2, 361-391 [3] Podgrabinska, S., Braun, P., Velasco, P., Kloos, B., Pepper, M., Jackson, D. and Skobe, M. (2002) Molecular characterization of lymphatic endothelial cells. PNAS 99:16069-16074. |
25001.) Speyer CL, Hachem AH, Assi AA, Johnson JS, DeVries JA, Gorski DH. (2014) "Metabotropic Glutamate Receptor-1 as a Novel Target for the Antiangiogenic Treatment of Breast Cancer." PloS one. 9: e88830.
2.) Yue X, Xu L, Zhu M, Zhang R, Liu C. (2013) "Over-expression of Nerve Growth Factor-? in human cholangiocarcinoma QBC939 cells promote tumor progression." PLoS One. 8: e62024.
3.) Park HS, Jung IM, Choi GH, Hahn S, Yoo YS, Lee T. (2013) 'Modification of a Rodent Hindlimb Model of Secondary Lymphedema: Surgical Radicality versus Radiotherapeutic Ablation.'
4.) Park HS, Jung IM, Choi GH, Hahn S, Yoo YS, Lee T. (2013) "Modification of a Rodent Hindlimb Model of Secondary Lymphedema: Surgical Radicality versus Radiotherapeutic Ablation." BioMed res int. 2013: 208912.
5.) Qiu Y, Chen Y, Fu X, Zhang L, Tian J, Hao Q. (2012) "HMGB1 promotes lymphangiogenesis of human lymphatic endothelial cells in vitro." Med Oncol. 29: 358-63.
6.) Bouta EM, McCarthy CW, Keim A, Wang HB, Gilbert RJ, Goldman J. (2011) "Biomaterial guides for lymphatic endothelial cell alignment and migration." Acta Biomater. 7: 1104-13.
7.) Hu J, Ye H, Fu A, Chen X, Wang Y, Chen X, Ye X, Xiao W, Duan X, Wei Y, Chen L. (2010) "Deguelin--an inhibitor to tumor lymphangiogenesis and lymphatic metastasis by downregulation of vascular endothelial cell growth factor-D in lung tumor model." Int J Cancer. 127: 2455-66.
8.) Zhuang Z, Jian P, Longjiang L, Bo H, Wenlin X. (2010) "Altered phenotype of lymphatic endothelial cells induced by highly metastatic OTSCC cells contributed to the lymphatic metastasis of OTSCC cells." Cancer Sci. 101: 686-92.
9.) Wen J, Fu AF, Chen LJ, Xie XJ, Yang GL, Chen XC, Wang YS, Li J, Chen P, Tang MH, Shao XM, Lu Y, Zhao X, Wei YQ. (2009) "Liposomal honokiol inhibits VEGF-D-induced lymphangiogenesis and metastasis in xenograft tumor model." Int J Cancer. 124: 2709-18.
10.) Thapa N, Hong HY, Sangeetha P, Kim IS, Yoo J, Rhee K, Oh GT, Kwon IC, Lee BH. (2008) "Identification of a peptide ligand recognizing dysfunctional endothelial cells for targeting atherosclerosis." J Control Release. 131: 27-33.
11.) Yoon CM, Hong BS, Moon HG, Lim S, Suh PG, Kim YK, Chae CB, Gho YS. (2008) "Sphingosine-1-phosphate promotes lymphangiogenesis by stimulating S1P1/Gi/PLC/Ca2+ signaling pathways." Blood. 112: 1129-38.
12.) Zhuang Z, Jian P, Longjiang L, Bo H, Hongwei Z. (2008) "Identification of oral cancer cell-induced changes in gene expression profile of lymphatic endothelial cell." Cancer Invest. 26: 1002-7.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1001 | ECM | Endothelial Cell Medium |
$139.00
As low as:
$92.00
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| 8248 | BPF | Bovine Plasma Fibronectin |
$137.00
|
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| MOLECULAR BIOLOGY | |||||
| 2504 | HLEC cDNA | Human Lymphatic Endothelial Cell cDNA |
$453.00
|
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| 2505 | HLEC tRNA | Human Lymphatic Endothelial Cell Total RNA |
$401.00
|
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| 2506 | HLEC Lysate | Human Lymphatic Endothelial Cell Lysate |
$438.00
|
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| 2507 | HLEC miRNA | Human Lymphatic Endothelial Cell MicroRNA |
$374.00
|
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| 2509 | HLEC gDNA | Human Lymphatic Endothelial Cell genomic DNA |
$438.00
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