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- Human Nucleus Pulposus Cells
Nucleus pulposus is the jelly-like substance in the middle of the intervertebral disc. It functions to distribute hydraulic pressure in all directions within each disc under compressive loads. The nucleus pulposus consists of collagen fibrils, proteoglycan aggrecans and nucleus pulposus cells. Nucleus pulposus cells reside in an environment that has a limited vascular supply and generate energy through anaerobic glycolysis. The cells synthesize collagen II and X; express hypoxia-inducible factor-1 [1], and secrete interleukins-1, -6, and -10 and granulocyte-macrophage colony-stimulating factor [2]. Mechanical stress on nucleus pulposus cells promotes cell proliferation [3]. Nucleus pulposus cells provide an in vitro model for the study of cellular and molecular events involved in disc degeneration, tissue engineering and cell therapy for spine disc disorders.
HNPC from ScienCell Research Laboratories are isolated from the nucleus pulposus of human intervertebral disc. HNPC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HNPC are characterized by immunofluorescence with antibodies specific to fibronectin and vimentin. HNPC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HNPC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Nucleus Pulposus Cell Medium (NPCM, Cat. #4801) for the culturing of HNPC in vitro.
| Catalog No. | 4800 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HNPC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1]. A Agrawal, A Guttapalli, S Narayan, TJ. Albert, IM. Shapiro, and MV. Risbud (2007) Normoxic stabilization of HIF-1? drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk. Am J Physiol Cell Physiol 293: C621-C631. [2]. K Torsten, N Thomas, G Christoph, G Tatiana (2005) Human Anulus Fibrosis and Nucleus Pulposus Cells of the Intervertebral Disc: Effect of Degeneration and Culture System on Cell Phenotype. Spine 30(24):2743-2748. [3]. M Takuji, K Mamoru, K Koichi, T Toru, T Tetsuya (1999) Cyclic Mechanical Stretch Stress Increases the Growth Rate and Collagen Synthesis of Nucleus Pulposus Cells In Vitro. Spine 24(4):315-319. |
1.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
2.) Wang, Z., Wang, G., Zhu, X., Geng, D. & Yang, H. (2015) Interleukin-2 is upregulated in patients with a prolapsed lumbar intervertebral disc and modulates cell proliferation, apoptosis and extracellular matrix metabolism of human nucleus pulposus cells Exp Ther Med. 10
3.) Foss BL, Maxwell TW, Deng Y. (2014). "Chondroprotective supplementation promotes the mechanical properties of injectable scaffold for human nucleus pulposus tissue engineering." J MechBehav Biomed Mater. 29: 56-67.
4.) Ehlicke F, Freimark D, Hei B, Dorresteijn A, Czermak P. (2011) "Intervertebral disc regeneration: influence of growth factors on differentiation of human mesenchymal stem cells (hMSC)." IntJ Artif Organs. 33: 244-52.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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4800| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 4801 | HNPC | Nucleus Pulposus Cell Medium |
$122.00
As low as:
$81.00
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| REAGENTS | |||||
| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
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| 0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
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| MOLECULAR BIOLOGY | |||||
| 4804 | HNPC cDNA | Human Nucleus Pulposus Cell cDNA |
$411.00
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| 4805 | HNPC tRNA | Human Nucleus Pulposus Cell Total RNA |
$364.00
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| 4806 | HNPC Lysate | Human Nucleus Pulposus Cell Lysate |
$398.00
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| 4807 | HNPC miRNA | Human Nucleus Pulposus Cell MicroRNA |
$340.00
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| 4809 | HNPC gDNA | Human Nucleus Pulposus Cell genomic DNA |
$398.00
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