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- Human Trabecular Meshwork Cells
Trabecular meshwork is located around the circumference of the root of the iris and has a slit opening into the Schlemm’s canal that drains aqueous to venous vessels. Trabecular meshwork cells (TMC) play an active role in the aqueous humor outflow and this pathway is intraocular pressure-dependent. Injury or death of TMC, therefore, has been associated with the pathogenesis of open-angle glaucoma. TMC further express receptors for neurotransmitters and neuropeptides, and respond to very low concentrations of a variety of vasoactive peptides and growth factors, allowing these cells to regulate the permeability of trabecular meshwork at multiple levels. TMC cultures provides an invaluable tool to study the functional control of trabecular meshwork, encouraging the development of more efficient strategies to treat glaucoma.
HTMC from ScienCell Research Laboratories are isolated from the juxtacanalicular and corneoscleral regions of human eye. HTMC are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HTMC are characterized by immunofluorescence with antibodies specific to α-smooth muscle actin and fibronectin. HTMC are negative for HIV- 1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HTMC are guaranteed to further expand for 15 population doublings under the conditions specified by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Trabecular Meshwork Cell Medium (TMCM, Cat. #6591) for culturing HTMC in vitro.
| Catalog No. | 6590 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HTMC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Stamer WD, Acott TS. (2012) "Current understanding of conventional outflow dysfunction in glaucoma." Curr Opin Ophthalmol. 23: 135-43.
[2] Llobet A, Gasull X, Gual A. (2003) "Understanding trabecular meshwork physiology: a key to the control of intraocular pressure?" News Physiol Sci. 18: 205-9. |
1.) Yu, N., Zhang, Z., Chen, P., Zhong, Y., Cai, X., Hu, H., Yang, Y., Zhang, J., Li, K., Ge, J., Yu, K., Liu, X. & Zhuang, J. (2015) Tetramethylpyrazine (TMP), an Active Ingredient of Chinese Herb Medicine Chuanxiong, Attenuates the Degeneration of Trabecular Meshwork through SDF-1/CXCR4 Axis PLoS One. 10
2.) Inoue-Mochita, M., Inoue, T., Fujimoto, T., Kameda, T., Awai-Kasaoka, N., Ohtsu, N., Kimoto, K. & Tanihara, H. (2015) p38 MAP Kinase Inhibitor Suppresses Transforming Growth Factor-?2?Induced Type 1 Collagen Production in Trabecular Meshwork Cells PLoS One. 10
3.) Diskin S, Chen WS, Cao Z, Gyawali S, Gong H, Soza A, Gonz?lez A, Panjwani N. (2012) "Galectin-8 promotes cytoskeletal rearrangement in trabecular meshwork cells through activation of Rho signaling." PLoS One. 7: e44400.
4.) Famili A, Ammar DA, Kahook MY. (2013) "Ethyl pyruvate treatment mitigates oxidative stress damage in cultured trabecular meshwork cells." Mol Vis. 19: 1304-9.
5.) Sacc? SC, La Maestra S, Micale RT, Larghero P, Travaini G, Baluce B, Izzotti A. (2011) "Ability of dorzolamide hydrochloride and timolol maleate to target mitochondria in glaucoma therapy." Arch Ophthalmol. 129: 48-55.
6.) Miyamoto N, Izumi H, Miyamoto R, Kondo H, Tawara A, Sasaguri Y, Kohno K. (2011) "Quercetin induces the expression of peroxiredoxins 3 and 5 via the Nrf2/NRF1 transcription pathway." Invest Ophthalmol Vis Sci. 52: 1055-63.
7.) Hudson BD, H?bert TE, Kelly ME. (2010) "Physical and functional interaction between CB1 cannabinoid receptors and beta2-adrenoceptors." Br J Pharmacol. 160: 627-42.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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6590| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 6591 | TMCM | Trabecular Meshwork Cell Medium |
$116.00
As low as:
$78.00
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| REAGENTS | |||||
| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
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| 0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
|
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| MOLECULAR BIOLOGY | |||||
| 6594 | HTMC cDNA | Human Trabecular Meshwork Cell cDNA |
$411.00
|
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| 6595 | HTMC tRNA | Human Trabecular Meshwork Cell Total RNA |
$364.00
|
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| 6596 | HTMC Lysate | Human Trabecular Meshwork Cell Lysate |
$398.00
|
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| 6597 | HTMC miRNA | Human Trabecular Meshwork Cell MicroRNA |
$340.00
|
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| 6599 | HTMC gDNA | Human Trabecular Meshwork Cell Genomic DNA |
$398.00
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