- Home /
- Human Umbilical Mesenchymal Stem Cells
Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. MSC have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties, in combination with their developmental plasticity, have generated tremendous interest because of the potential use of MSC in regenerative medicine. MSC isolated from the Wharton’s jelly of the umbilical cord were induced to transform into neurons and glia in vitro by using neuron-conditioned medium, sonic hedgehog, and FGF-8. MSC can also differentiate into cells from the adipogenic and osteogenic lineage. Additionally, they have the potential to differentiate into cardiomyocytes by culturing them in cardiomyocyte-conditioned medium. MSC express the matrix receptors CD44 and CD105 and mesenchymal stem cell markers SH2 and SH3, but not the hematopoietic lineage marker CD34.
HUMSC from ScienCell Research Laboratories are isolated from Wharton’s jelly of the umbilical cord. HUMSC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HUMSC are characterized by immunofluorescence with antibodies specific to CD73 and/or CD90, Oil Red O staining after adipogenic differentiation, and Alizarin Red staining after osteogenic differentiation. HUMSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUMSC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. #7501) for culturing HUMSC in vitro.
| Catalog No. | 7530 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HUMSC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Mitchell, K. E. et al. (2003) Matrix cells from Wharton's jelly form neurons and glia. Stem Cells 21:50-60. [2] Fu, Y. S. et al. (2006) Conversion of human umbilical cord mesenchymal stem cells in Wharton's jelly to dopaminergic neurons in vitro: potential therapeutic application for Parkinsonium. Stem cells 24:115-124. [3] Wang, H. S., et al., (2004) Mesenchymal stem cells in the Wharton's jelly of the human umbilical cord. Stem cells 22:1330-1337. |
75301.) Liu J, Xu HH, Zhou H, Weir MD, Chen Q, Trotman CA. (2013) "Human umbilical cord stem cell encapsulation in novel macroporous and injectable fibrin for muscle tissue engineering." Acta Biomater. 9: 4688-97.
2.) Lee HK, Finniss S, Cazacu S, Bucris E, Ziv-Av A, Xiang C, Bobbitt K, Rempel SA, Hasselbach L, Mikkelsen T, Slavin S, Brodie C. (2013) "Mesenchymal stem cells deliver synthetic microRNA mimics to glioma cells and glioma stem cells and inhibit their cell migration and self-renewal." Oncotarget. 4: 346-61.
3.) Chen W, Zhou H, Tang M, Weir MD, Bao C, Xu HH. (2012) "Gas-foaming calcium phosphate cement scaffold encapsulating human umbilical cord stem cells." Tissue Eng Part A. 18: 816-27.
4.) Liu J, Zhou H, Weir MD, Xu HH, Chen Q, Trotman CA. (2012) "Fast-degradable microbeads encapsulating human umbilical cord stem cells in alginate for muscle tissue engineering." Tissue Eng Part A. 18: 2303-14.
5.) Tang M, Weir MD, Xu HH. (2012) "Mannitol-containing macroporous calcium phosphate cement encapsulating human umbilical cord stem cells." J Tissue Eng Regen Med. 6: 214-24.
6.) Theinhan W, Weir MD, Simon CG, Xu HH. (2012) "Non-rigid calcium phosphate cement containing hydrogel microbeads and absorbable fibres seeded with umbilical cord stem cells for bone engineering." J Tissue Eng Regen Med. doi: 10.1002/term.1466.
7.) Zhou H, Chen W, Weir MD, Xu HH. (2012) "Biofunctionalized calcium phosphate cement to enhance the attachment and osteodifferentiation of stem cells released from fast-degradable alginate-fibrin microbeads." Tissue Eng Part A. 18: 1583-95.
8.) Thein-Han W, Xu HH. (2011) "Collagen-calcium phosphate cement scaffolds seeded with umbilical cord stem cells for bone tissue engineering." Tissue Eng Part A. 17: 2943-54.
9.) Zhao Y, Yu L, Xu S, Qiu F, Fan Y, Fu G. (2011) "Down-regulation of connexin43 gap junction by serum deprivation in human endothelial cells was improved by (-)-Epigallocatechin gallate via ERK MAP kinase pathway." Biochem Biophys Res Commun. 404: 217-22.
10.) Zhao Z, Chen Z, Zhao X, Pan F, Cai M, Wang T, Zhang H, Lu JR, Lei M. (2011) "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions." J Biomed Sci. 18: 37.
11.) Zhou H, Weir MD, Xu HH. (2011) "Effect of cell seeding density on proliferation and osteodifferentiation of umbilical cord stem cells on calcium phosphate cement-fiber scaffold." Tissue Eng Part A. 17: 2603-13.
12.) Zhou H, Xu HH. (2011) "The fast release of stem cells from alginate-fibrin microbeads in injectable scaffolds for bone tissue engineering." Biomaterials. 32: 7503-13.
13.) Peng J, Wang Y, Zhang L, Zhao B, Zhao Z, Chen J, Guo Q, Liu S, Sui X, Xu W, Lu S. (2011) "Human umbilical cord Wharton's jelly-derived mesenchymal stem cells differentiate into a Schwann-cell phenotype and promote neurite outgrowth in vitro." Brain Res Bull. 84: 235-43.
14.) Zhao L, Burguera EF, Xu HH, Amin N, Ryou H, Arola DD. (2010) "Fatigue and human umbilical cord stem cell seeding characteristics of calcium phosphate-chitosan-biodegradable fiber scaffolds." Biomaterials. 31: 840-7.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 7140 | HAMSC | Human Amniotic Mesenchymal Stromal Cells |
$738.00
|
||
| 7150 | HCMSC | Human Chorionic Mesenchymal Stromal Cells |
$738.00
|
||
| 7500 | HMSC-bm | Human Bone Marrow-derived Mesenchymal Stem Cells |
$738.00
|
||
| 7510 | HMSC-ad | Human Adipose-derived Mesenchymal Stem Cells (HMSC-ad) |
$738.00
|
||
| 7520 | HMSC-he | Human Liver-derived Mesenchymal Stem Cells |
$738.00
|
||
| 7540 | HPMSC | Human Pulmonary Mesenchymal Stem Cells |
$738.00
|
||
| 7550 | HVMSC | Human Vertebral Mesenchymal Stem Cells |
$738.00
|
||
| REAGENTS | |||||
| 0843 | ORed | Oil Red O Staining Kit |
$100.00
|
||
| 8298 | HMSC-A-qPCR | Human Mesenchymal Stem Cell Adipogenesis Detection qPCR Kit |
$282.00
|
||
| 0223 | ARed | Alizarin Red S Staining Kit |
$78.00
|
||
| 8328 | HMSC-C-qPCR | Human Mesenchymal Stem Cell Chondrogenesis Detection qPCR Kit |
$282.00
|
||
| 8348 | SafraninO | Safranin O Staining Kit |
$106.00
|
||
| 8378 | ABlue | Alcian Blue Staining Kit |
$106.00
|
||
| MEDIA | |||||
| 7501 | MSCM | Mesenchymal Stem Cell Medium |
$126.00
As low as:
$81.00
|
||
| 7511 | MSCM-sf | Mesenchymal Stem Cell Medium-serum free |
$208.00
As low as:
$104.00
|
||
| 7521 | MSCM-acf | Mesenchymal Stem Cell Medium-animal component free |
$232.00
As low as:
$115.00
|
||
| 7551 | MCDM | Mesenchymal Stem Cell Chondrogenic Differentiation Medium |
$284.00
As low as:
$104.00
|
||
| 7531 | MODM | Mesenchymal Stem Cell Osteogenic Differentiation Medium |
$336.00
As low as:
$104.00
|
||
| 7541 | MADM | Mesenchymal Stem Cell Adipogenic Differentiation Medium |
$349.00
|
||
| 8678 | ARed-Q | Alizarin Red S Staining Quantification Assay |
$170.00
|
||
| 0933 | MesenF | MesenFectagen |
$253.00
|
||
| MOLECULAR BIOLOGY | |||||
| 7536 | HUMSC Lysate | Human Umbilical Mesenchymal Stem Cell Lysate |
$398.00
|
||
| 7537 | HUMSC miRNA | Human Umbilical Mesenchymal Stem Cell MicroRNA |
$340.00
|
||
| 7539 | HUMSC gDNA | Human Umbilical Mesenchymal Stem Cell genomic DNA |
$398.00
|
||
| 7535 | HUMSC tRNA | Human Umbilical Mesenchymal Stem Cell Total RNA |
$364.00
|
||
| 7534 | HUMSC cDNA | Human Umbilical Mesenchymal Stem Cell cDNA |
$411.00
|
||
