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- Insulin Transferrin Selenium, 10 ml
ITS is a mixture of insulin, transferrin, and selenium. It is a general cell supplement designed for use in classical media such as DMEM, RPMI-1640 and nutrient media such as Ham’s F-12, DMEM/F-12. ITS enhances cell proliferation and decreases the serum requirement of many cell types [1, 2, 3]. When ITS is used with a low percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.
ITS is prepared in Hank’s Balanced Salt Solution (HBSS) without phenol red.
Insulin 1.0 mg/ml
Apo-Transferrin 1.0 mg/ml
Sodium Selenite 3.4 µM
|Country of Manufacture||United States|
|Product Use||THESE PRODUCTS ARE FOR RESEARCH USE ONLY. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro procedures.|
|Shipping Info||Dry ice.|
|References||1. Guilbert, L. J. and Iscove, N. N. (1976) Partial replacement of serum be selenite, transferrin, albumin, and lecithin onhaemopoietic cell culture. Nature, 263: 594-595.
2. Kelley, D. S., Becker, J. E., and Porter, V. R. (1978) Effects of insulin, dexamethasone, and glucagon on the amino acid transport ability of four rat hepatoma cell lines and rat hepatocytes in culture. Cancer Res., 38: 4591- 4601. s, NY, p. 103.
3. McKeehan, W. L., Hamilton, W. G., and Ham, R. G. (1976) Selenium is an essential trace nutrient for the growth of WI-38 diploid human fibroblasts. Proc. Natl. Acad. Sci., USA 73: 2023-2027.
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1.) Gambardella G, Moretti MN, de Cegli R, Cardone L, Peron A, di Bernardo D. (2013) "Differential network analysis for the identification of condition-specific pathway activity and regulation." Bioinformatics. 29: 1776-85.
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