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How can we help you today?
Need help with product selection and ordering?
Email us at sales@sciencellonline.com
Need help with scientific inquiries, technical assistance, product applications, and use?
Email us at techsupport@sciencellonline.com
Need help with issues concerning this website?
Email us at webmaster@sciencellonline.com
Have any marketing related inquiries?
Email us at marketing@sciencellonline.com
Need help ordering from abroad?
As the largest cell provider, we are available worldwide.
Please visit our Distributors page to select the company nearest to your location.
We are located at 1610 Faraday Ave, Carlsbad, CA 92008
Call us at (877) 602-8549 or (760) 602-8549
Fax us at (760) 602-8575
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The most abundant cell type in the lung interstitium is the fibroblast. Pulmonary fibroblasts (PF) exhibit typical fibroblast morphology, however, they also possess some distinguishing features such as long, branching processes and gap junctions. PF function to produce type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa. PF also play an important role in the repair and remodeling processes following tissue damage. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury. An inadequate or an excessive accumulation of fibroblasts results in abnormal tissue function and can lead to the development of pulmonary fibrosis. Notably, pulmonary fibrosis has been observed in acute COVID-19 patients, however, little is known about how the fibrosis will affect lung function in recovered patients. The culture of mouse PF (MPF) in vitro can be used to further elucidate the mechanisms of pulmonary fibrosis and other types of lung disorders.
MPF from ScienCell Research Laboratories are isolated from CD-1 IGS mouse lung. MPF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MPF are characterized by immunofluorescence with antibody specific to fibronectin. MPF are negative for mycoplasma, bacteria, yeast, and fungi. MPF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing MPF in vitro.
Catalog No. | M3300 |
---|---|
Country of Manufacture | United States |
Product Code | MPF |
Size/Quantity | 5 x 10^5 cells/vial |
Product Use | MPF are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
Shipping Info | Dry ice. |
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