Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding to its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand (FasL) is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the target cell. This usually leads to apoptosis. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.
ScienCell's human FASL ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human FASL-specific monoclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human FASL captured in strips.
|Country of Manufacture||United States|
|Product Use||This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.|
|Storage||Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.|
|Shipping Info||Shipped on gel ice.|
|References||1. Asanuma, K., Tsuji, N., Endoh, T., Yagihashi, A., Watanabe, N.Survivin enhances Fas ligand expression via up-regulation of specificity protein 1-mediated gene transcription in colon cancer cells.J. Immun. 172: 3922-3929, 2004.
2. Hahne, M., Rimoldi, D., Schroter, M., Romero, P., Schreier, M., French, L. E., Schneider, P., Bornand, T., Fontana, A., Lienard, D., Cerottini, J. C., Tschopp, J.Melanoma cell expression of Fas (Apo-1/CD95) ligand: implications for tumor immune escape.Science 274: 1363-1366, 1996.
3. Nakamura, T., Imai, Y., Matsumoto, T., Sato, S., Takeuchi, K., Igarashi, K., Harada, Y., Azuma, Y., Krust, A., Yamamoto, Y., Nishina, H., Takeda, S., Takayanagi, H., Metzger, D., Kanno, J., Takaoka, K., Martin, T. J., Chambon, P., Kato, S.Estrogen prevents bone loss via estrogen receptor alpha and induction of Fas ligand in osteoclasts.Cell 130: 811-823, 2007.
Are you a regular user of ScienCell products? If so, tell us how you like our products by writing a review and receive a discount for your future purchase.
Has your paper been published using one or more of Sciencell products? Get rewarded! Receive 10% off one order*. See details >>
No Publication available at this time
Certificate of Analysis
Certificate of Analysis for Human FASL ELISA Kit does not exist
For specific lot number search below:
Click on Questions to view answers:
There are no product questions yet.