Recombinant Murine FGF

Basic Fibroblast Growth Factor (bFGF) is a member of the FGF family of mitogenic peptides which is comprised of at least 22 proteins showing 35-55% amino acid sequence conservation. Unlike other FGF family members, bFGF and acidic FGF (aFGF) lack signal peptides and are secreted via a different mechanism other than the classical protein secretion pathway. The predicted 17 kDa bFGF isoform can be located in both the cytoplasm and the nucleus. The use of alternative transcription start sites within the gene produces several different isoforms ranging from 21-24kDa proteins.

Catalog No.	124-02
Country of Manufacture	United States or China
Product Code	rmbFGF
Size/Quantity	10µg
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Store at -20°C after receiving. Upon reconstitution, store at 2-8°C for up to one week. For maximal stability, aliquot and store at -20°C. Avoid repeated freeze/ thaw cycles.
Shipping Info	Dry ice.
Synonyms	FGF-2, Fibroblast Growth Factor-basic
AA Sequence	MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS
Source	Escherichia coli
Molecular Weight	Approximately 16.2 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.
Purity	>98% by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active. The ED₅₀ is < 0.1ng/ml, corresponding to a specific activity of 1 x 10^7 units/mg, as determined by proliferation of BaF3 cells expressing FGF receptors
Physical Appearance	White lyophilized powder.
Formulation	Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.
Endotoxin	< 1EU/μg of growth factor as determined by LAL method.
Reconstitution	Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.