- Home /
- Product & Services /
- Primary Cells /
- Human /
- Cell Systems /
- Pulmonary Cell System /
- Human Pulmonary Artery Smooth Muscle Cells
Smooth muscle cells (SMC) are primary contributors to the development of arterial disease. The ability of vascular SMC to switch to a proliferative phenotype is one of the main factors in the development and progression of vascular disease. Pulmonary artery smooth muscle cells (PASMC) express VEGF and FGF-2 and are subjected to mechanical forces during pulsatile blood flow. Chronic lung hypoxia causes vascular remodeling with PASMC hyperplasia, and results in pulmonary hypertension. Cultured PASMC play an important role in vascular disease research and can be used to identify new therapeutic targets to treat pulmonary vascular disease.
HPASMC from ScienCell Research Laboratories are isolated from human pulmonary arteries. HPASMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HPASMC are characterized by immunofluorescence with antibodies specific to α-smooth muscle actin. HPASMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPASMC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. #1101) for culturing HPASMC in vitro.
Catalog No. | 3110 |
---|---|
Country of Manufacture | United States |
Product Code | HPASMC |
Size/Quantity | 5 x 10⁵ cells/vial. |
Product Use | HPASMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Schwartz, S. M., Campbell, G. R., Campbell, J. H. (1986) Replication of smooth muscle cells in vascular disease. Circ. Res. 58:427-444. [2] Fan, Q. I., Vanderpool, K., Marsh, J. D. (2002) A 27 bp cis-acting sequence is essential for L-type calcium channel alpha(1C) subunit expression in vascular smooth muscle cells. Biochim Biophys Acta. 1577:401-11. [3] Braun, M., Pietsch, P., Schror, K., Baumann, G., Felix, S. B. (1999) Cellular adhesion molecules on vascular smooth muscle cells. Cardiovasc. Res. 41:395-401. [4] Quinn, T. P., Schlueter, M., Soifer, S. J., Gutierrez, J. A. (2002) Cyclic mechanical stretch induces VEGF and FGF-2 Expression in pulmonary vascular smooth muscle cells. Am. J. Physiol. Lung Cell Mol. Physiol. 282:L897-903. [5] Rose, F., et al. (2002) Hypoxic pulmonary artery fibroblasts trigger proliferation of vascular smooth muscle cells: role of hypoxia-inducible transcription factors. FASEB J. 12:1660-1. |
1.) Zheng, Y., Ma, H., Yan, Y., Ye, P., Yu, W., Lin, S., & Chen, S.-L. (2023). Deficiency of filamin a in smooth muscle cells protects against hypoxia mediated pulmonary hypertension in mice. International Journal of Molecular Medicine, 51(3). https://doi.org/10.3892/ijmm.2023.5225
2.) Zhang, S., Liu, B., Fan, Z., Wang, D., Liu, Y., Li, J., Wang, N., Liu, Y. & Zhang, B. (2016) Targeted inhibition of survivin with YM155 promotes apoptosis of hypoxic human pulmonary arterial smooth muscle cells via the upregulation of voltage-dependent K+ channels Mol Med Rep. 13
3.) Li, Q., Yan, X., Kong, H., Xie, W. & Wang, H.(2016) 'Iptakalim influences the proliferation and apoptosis of human pulmonary artery smooth muscle cells' Mol Med Rep. VOL 14
4.) Garc?a alvarez, A., Pereda, D., Garc?aLunar, I., SanzRosa, D., Fern?ndezJim?nez, R., Garc?aPrieto, J., Nu?oAyala, M., Sierra, F., Santiago, E., Sandoval, E., Campelos, P., Ag?ero, J., Pizarro, G., Peinado, V.I., Fern?ndezFriera, L., Garc?aRuiz, J.M., Barber?, J.A., Castell?, M., Sabat?, M., Fuster, V. & Iba?ez, B.(2016) 'Beta3 adrenergic agonists reduce pulmonary vascular resistance and improve right ventricular performance in a porcine model of chronic pulmonary hypertension' Basic Research in Cardiology. VOL 111
5.) Yuan, K., Orcholski, M.E., Panaroni, C., Shuffle, E.M., Huang, N.F., Jiang, X., Tian, W., Vladar, E.K., Wang, L., Nicolls, M.R., Wu, J.Y. & de Jesus Perez, V.A. (2015) Activation of the Wnt/Planar Cell Polarity Pathway Is Required for Pericyte Recruitment during Pulmonary Angiogenesis Am J Pathol. 185
6.) Wang HL, Peng LP, Chen WJ, Tang SH, Sun BZ, Wang CL, Huang R, Xu ZJ, Lei WF. (2014) 'HMGB1 enhances smooth muscle cell proliferation and migration in pulmonary artery remodeling.'
7.) Ibrahim YF, Wong CM, Pavlickova L, Liu L, Trasar L, Bansal G, Suzuki YJ. (2014) 'Mechanism of the Susceptibility of Remodeled Pulmonary Vessels to Drug?Induced Cell Killing.'
8.) Wang G, Liu X, Meng L, Liu S, Wang L, Li J, Cui C, Meng J, Hu S, Wei Y. (2014) 'Up-Regulated Lipocalin-2 in Pulmonary Hypertension Involving in Pulmonary Artery SMC Resistance to Apoptosis.'
9.) Ibrahim YF, Wong CM, Pavlickova L, Liu L, Trasar L, Bansal G, Suzuki YJ. (2014) "Mechanism of the Susceptibility of Remodeled Pulmonary Vessels to Drug?Induced Cell Killing." J Am Heart Assoc. 3: e000520.
10.) Wang G, Liu X, Meng L, Liu S, Wang L, Li J, Cui C, Meng J, Hu S, Wei Y. (2014) "Up-Regulated Lipocalin-2 in Pulmonary Hypertension Involving in Pulmonary Artery SMC Resistance to Apoptosis." Int j biol sci. 10: 798-806.
11.) Wang HL, Peng LP, Chen WJ, Tang SH, Sun BZ, Wang CL, Huang R, Xu ZJ, Lei WF. (2014) "HMGB1 enhances smooth muscle cell proliferation and migration in pulmonary artery remodeling." Int j clinexppathol. 7: 3836-44.
12.) Zha WJ, Qian Y, Shen Y, Du Q, Chen FF, Wu ZZ, Li X, Huang M. (2013) "Galangin Abrogates Ovalbumin-Induced Airway Inflammation via Negative Regulation of NF-?B." Evid Based Complement Alternat Med. 2013:767689.
13.) Wang G, Zhang Z, Xu Z, Yin H, Bai L, Ma Z, Decoster MA, Qian G, Wu G. (2010) "Activation of the sonic hedgehog signaling controls human pulmonary arterial smooth muscle cell proliferation in response to hypoxia." Biochim Biophys Acta. 1803: 1359-67.
14.) de Frutos S, Nitta CH, Caldwell E, Friedman J, Gonz?lez Bosc LV. (2009) "Regulation of soluble guanylyl cyclase-alpha1 expression in chronic hypoxia-induced pulmonary hypertension: role of NFATc3 and HuR." Am J Physiol Lung Cell Mol Physiol. 297: L475-86.
15.) de Jesus Perez VA, Alastalo TP, Wu JC, Axelrod JD, Cooke JP, Amieva M, Rabinovitch M. (2009) "Bone morphogenetic protein 2 induces pulmonary angiogenesis via Wnt-beta-catenin and Wnt-RhoA-Rac1 pathways." J Cell Biol.184: 83-99.
16.) Wang Z, Yuan Z, Jin L. (2008) "Gene delivery into hepatocytes with the preS/liposome/DNA system." Biotechnol J. 3: 1286-95.
17.) Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL. (2007) "Evolving the lock to fit the key to create a family of G protein-coupled receptors potently activated by an inert ligand." Proc Natl Acad Sci USA. 104: 5163-8.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
---|---|---|---|---|---|
MEDIA | |||||
1101 | SMCM | Smooth Muscle Cell Medium |
$126.00
As low as:
$86.00
|
||
MOLECULAR BIOLOGY | |||||
3114 | HPASMC cDNA | Human Pulmonary Artery Smooth Muscle Cell cDNA |
$453.00
|
||
3115 | HPASMC tRNA | Human Pulmonary Artery Smooth Muscle Cell Total RNA |
$401.00
|
||
3116 | HPASMC Lysate | Human Pulmonary Artery Smooth Muscle Cell Lysate |
$438.00
|
||
3117 | HPASMC miRNA | Human Pulmonary Artery Smooth Muscle Cell MicroRNA |
$374.00
|
||
3119 | HPASMC gDNA | Human Pulmonary Artery Smooth Muscle Cell genomic DNA |
$438.00
|