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The pulmonary microvascular endothelial cells (PMEC) lining the pulmonary vasculature form a semiselective barrier which is critical for gas exchange and the regulation of fluid, macromolecules, and cells between the blood and the interstitial compartments in the lung. PMEC also produce angiotensin-converting enzyme to activate angiotensin, a peptide hormone which causes vasoconstriction and increases blood pressure. PMEC further plays an important role in modulating cellular properties of pulmonary mesenchymal cells and inflammatory events. Such functions of PMEC are implicated in acute lung injury, pulmonary hypertension, edema, embolism, and lung cancer development.
HPMEC from ScienCell Research Laboratories are isolated from human lung. HPMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HPMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM). HPMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HPMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HPMEC in vitro.
Catalog No. | 3000 |
---|---|
Country of Manufacture | United States |
Product Code | HPMEC |
Size/Quantity | 5 x 10⁵ cells/vial. |
Product Use | HPMEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Brigham, K. L. (1990) Oxidant stress and adult respiratory distress syndrome. Eur Respir J Suppl 11: 482s-484s. [2] Moore T. M., Chetham P. M., Kelly J. J., Stevens T. (1998) Signal transduction and regulation of lung endothelial cell permeability. Interaction between calcium and cAMP. Am J Physiol. 275(2 Pt 1):L203-22. [3] Kelly JJ, Moore TM, Babal P, Diwan AH, Stevens T, Thompson WJ. (1998) Pulmonary microvascular and macrovascular endothelial cells: differential regulation of Ca2+ and permeability. Am J Physiol. 274(5 Pt 1):L810-9. |
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3.) Lamichhane, S.P., Arya, N., Ojha, N., Kohler, E. & Shastri, V.P. (2015) Glycosaminoglycan-functionalized poly-lactide-co-glycolide nanoparticles: synthesis, characterization, cytocompatibility, and cellular uptake Int J Nanomedicine. 10
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ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
---|---|---|---|---|---|
MEDIA | |||||
1001 | ECM | Endothelial Cell Medium |
$139.00
As low as:
$92.00
|
||
8248 | BPF | Bovine Plasma Fibronectin |
$137.00
|
||
MOLECULAR BIOLOGY | |||||
3004 | HPMEC cDNA | Human Pulmonary Microvascular Endothelial Cell cDNA |
$453.00
|
||
3005 | HPMEC tRNA | Human Pulmonary Microvascular Endothelial Cell Total RNA |
$401.00
|
||
3006 | HPMEC Lysate | Human Pulmonary Microvascular Endothelial Cell Lysate |
$438.00
|
||
3007 | HPMEC miRNA | Human Pulmonary Microvascular Endothelial Cell MicroRNA |
$374.00
|
||
3009 | HPMEC gDNA | Human Pulmonary Microvascular Endothelial Cell genomic DNA |
$438.00
|