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- Human Cerebellar Granule Cells
The development of the cerebellum involves a set of coordinated cell movements and two separate proliferation zones: the ventricular zone and the external granule cell layer (EGL), a rhombic-lip-derived progenitor pool. The EGL appears segregated during early cerebellum formation and produces only granule cells. Cerebellar granule cells (CGC) are the most abundant neurons in the brain, about 1 x 10^11 in humans. Their axons run as parallel fibres along the coronal axis, and the one-dimensional spread of excitation that results from this arrangement is a key assumption in theories of cerebellar function. CGC receive inhibitory synaptic input from Golgi cells, which are mediated by gamma-aminobutyric acid (GABA). During both in vivo and in vitro development, CGC depend on the activity of the NMDA glutamate receptor subtype for survival and full differentiation. Cultured CGC are widely used as a model system for studying neuronal apoptosis.
HCGC from ScienCell Research Laboratories are isolated from human cerebellum. HCGC are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HCGC are characterized by immunofluorescence with antibodies specific to β-tubulin III. HCGC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCGC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HCGC are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.
Recommended Medium
It is recommended to use Neuronal Medium (NM, Cat. #1521) for culturing HCGC in vitro.
| Catalog No. | 1530 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HCGC |
| Size/Quantity | 1 x 10^6 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Hatten, M. E. (1999) Central nervous system neuronal migration. Annu. Rev. Neurosci. 22, pp. 511-539. [2] Andersen, B.B., Korbo, L. and Pakkenberg, B. (1992) A quantitative study of the human cerebellum with unbiased stereological techniques. J. Comp. Neurol., 326:549-560. [3] Monti, B, Marri, L, Contestabile, A. (2002) NMDA receptor-dependent CREB activation in survival of cerebellar granule cells during in vivo and in vitro development. Eur J Neurosci. 16:1490-8. |
15301.) Ohno-Matsui K, Mori K, Ichinose S, Sato T, Wang J, Shimada N, Kojima A, Mochizuki M, Morita I. (2006) "In vitro and in vivo characterization of iris pigment epithelial cells cultured on aminotic membranes." Molecular Vision, 12: 1022-32.
2.) Sui Z, Fan S, Sniderhan L, Reisinger E, Litzburg A, Schifitto G, Gelbard HA, Dewhurst S, Maggirwar SB. (2006) "Inhibition of mixed lineage kinase 3 prevents HIV-1 Tat-mediated neurotoxicity and monocyte activation." J Immunol. 177: 702-11.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1521 | NM | Neuronal Medium |
$126.00
As low as:
$81.00
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| REAGENTS | |||||
| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
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| MOLECULAR BIOLOGY | |||||
| 1539 | HCGC gDNA | Human Cerebellar Granule Cell genomic DNA |
$398.00
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