The Interleukin 1 (IL-1) family is a group of 11 β trefoil cytokines. IL-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. Interleukin-1β (IL-1β) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β belongs to proinflammatory cytokines; promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1 on hepatic sinusoidal endothelium. ScienCell's rat IL-1β ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat IL-1β-specific monoclonal antibodies are pre-coated onto 8 x 12 divisible strips. The rat specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat IL-1β captured in wells.