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- Rat Renal Mesangial Cells
Renal mesangial cells are perivascular cells located within the central portion of the glomerular tuft between capillary loops, constituting 30-40% of the total glomerular cell population. They regulate the intraglomerular capillary flow and ultrafiltration surface via mesangial cell contraction and release of growth factors and vasoactive agents. By pinocytosis and phagocytosis, mesangial cells remove local accumulation of macromolecules in the mesangial space. Mesangial cells also synthesize, assemble and control turnover of the mesangial matrix. Overproduction of mesangial cells has been observed in various glomerular diseases, such as IgA nephropathy, mesangioproliferative glomerulonephritis, lupus nephritis, glomerulosclerosis and diabetic nephropathy. Such critical involvements suggest renal mesangial cells are an ideal model for studying mesangial injury and glomerular functions under both physiological and pathophysiological conditions.
RRMC from ScienCell Research Laboratories are isolated from rat kidney. RRMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RRMC are characterized by immunofluorescence with antibodies specific to fibronectin and/or smooth muscle actin. RRMC are negative for mycoplasma, bacteria, yeast, and fungi. RRMC are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Mesangial Cell Medium (MCM, Cat. #4201) for culturing RRMC in vitro.
| Catalog No. | R4200 |
|---|---|
| Country of Manufacture | United States |
| Product Code | RRMC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Olivetti G, Anversa P, Rigamonti W, et al. (1977) J Cell Biol. 75(2 Pt 1):573-85. [2] Couchman JR, Beavan LA and McCarthy KJ. (1994) Kidney Int. 45:328-35. [3] Riser BL, Cortes P, Heilig C, et al. (1996) Am J Pathol. 148:1915-23. [4] Gruden G, Thomas S, Burt D, et al. (1999) J Am Soc Nephrol 10:730-37. [5] Wanner C, Greiber S, Kramer-Guth A, et al. (1997) Kidney Int Suppl 63:S102-6. [6] Gomez-Guerrero C, Suzuki Y and Egido J. (2002) Kidney Int 62:715-7. [7] Schl |
R4200No Publication available at this time
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 4201 | MCM | Mesangial Cell Medium |
$122.00
As low as:
$81.00
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| REAGENTS | |||||
| 0103 | T/E | Trypsin/EDTA Solution (0.25%; 100ml) |
$21.00
|
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
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| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
|
